| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,221,135 | T→C | 15.4% | T253A (ACC→GCC) | yehY ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,221,135 | 0 | T | C | 15.4% | 93.0 / 10.9 | 39 | T253A (ACC→GCC) | yehY | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (18/15); new base C (4/2); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCACCAGCGCAATCAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCAACA > W3110S.gb/2221071‑2221203 | aCGCCGCGCACCAGCGGCAGCAAGGCATAGAGCACCAGCGCAATCAGTGCGGGTGTCATTCCGGTTCCTGc > 1:3020660/1‑71 (MQ=255) cATAGAGCACCAGCGCAATCAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGa < 1:3444429/71‑1 (MQ=255) tAGAGCACCAGCGCAATCAGTGCGGGTGTCATTCCGGCTCCTGCTATg > 1:3507465/1‑48 (MQ=255) tAGAGCACCAGCGCAATCAGTGCGGGTGTCATTCCGGCTCCTGCTATGCCGAGCGTCCCCAGCCACGGa > 1:3030026/1‑69 (MQ=255) gagCACCAGCGCAATCAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGaaa > 1:2135355/1‑69 (MQ=255) gagCACCAGCGCAATCAGTGCGGGTGTCATTCCGGTTACTGCTATGCCGAGCGTCCCCAGc > 1:3768465/1‑61 (MQ=255) aGCGCAATCAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAAc > 1:926032/1‑71 (MQ=255) aGCGCAATCAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAAc < 1:2135785/71‑1 (MQ=255) aGCGCAATCAGTGCGGGTGTCATTACGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTaa > 1:948171/1‑70 (MQ=255) cAATCAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAgc > 1:2227521/1‑71 (MQ=255) aTCAGTGCGGGTGTCATTCCGGCTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAgcgc > 1:2778766/1‑71 (MQ=255) cAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCgg > 1:1552876/1‑71 (MQ=255) gTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCa < 1:199851/71‑1 (MQ=255) gTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCa < 1:627266/71‑1 (MQ=255) cGGGTGTCATTCCGGCTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAgc < 1:468328/71‑1 (MQ=255) tgtCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGa < 1:830280/43‑1 (MQ=255) tgtCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGa < 1:1204837/43‑1 (MQ=255) tgtCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGa < 1:1404247/43‑1 (MQ=255) tgtCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAgcggcg > 1:1737332/1‑71 (MQ=255) gtCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAgcggcg > 1:1472557/1‑70 (MQ=255) tCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGgcgc > 1:2758707/1‑70 (MQ=255) tCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGgcgc > 1:968351/1‑70 (MQ=255) aTTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAAt > 1:2688989/1‑71 (MQ=255) aTTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAAt > 1:864448/1‑71 (MQ=255) aTTCCGGCTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAAt > 1:932733/1‑71 (MQ=255) ttCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATc < 1:102757/71‑1 (MQ=255) ttCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATc < 1:2432417/71‑1 (MQ=255) ttCCGGCTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATc < 1:3075690/71‑1 (MQ=255) ccGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa > 1:3527770/1‑71 (MQ=255) ccGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa > 1:3892076/1‑71 (MQ=255) ccGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa > 1:3034363/1‑71 (MQ=255) ccGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa > 1:961721/1‑71 (MQ=255) ccGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCa < 1:719058/70‑1 (MQ=255) ccGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCACGGCAAGCGGCGCAATCaa > 1:3417761/1‑71 (MQ=255) cGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa < 1:1891156/70‑1 (MQ=255) cGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa < 1:3583840/70‑1 (MQ=255) cGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa < 1:1304762/70‑1 (MQ=255) cGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCaa < 1:1300065/70‑1 (MQ=255) ggTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCAACa < 1:1456027/71‑1 (MQ=255) | ACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCACCAGCGCAATCAGTGCGGGTGTCATTCCGGTTCCTGCTATGCCGAGCGTCCCCAGCCACGGAAAGGCCGTAACCAGCGCGGCAAGCGGCGCAATCAACA > W3110S.gb/2221071‑2221203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |