| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,274,249 | A→G | 23.5% | T64A (ACT→GCT) | rtn → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,274,249 | 0 | A | G | 23.5% | 59.3 / 17.7 | 34 | T64A (ACT→GCT) | rtn | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (11/15); new base G (5/3); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GTCAAATACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGC > W3110S.gb/2274180‑2274313 | gTCAAATACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCac < 1:2739517/71‑1 (MQ=255) tCAAATACGACACACTGATTACAGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCac > 1:3041412/1‑70 (MQ=255) aTACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTg < 1:2789545/71‑1 (MQ=255) acTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTc > 1:3917373/1‑70 (MQ=255) gATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAg > 1:2046083/1‑70 (MQ=255) aTTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGc < 1:1566261/70‑1 (MQ=255) aTTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGc < 1:1610517/70‑1 (MQ=255) cGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCt < 1:1062662/71‑1 (MQ=255) tACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:3820087/71‑1 (MQ=255) tACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:2870504/71‑1 (MQ=255) tACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:267159/71‑1 (MQ=255) cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCt > 1:1362823/1‑67 (MQ=255) cAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCt > 1:2736059/1‑67 (MQ=255) tctcGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:406589/61‑1 (MQ=255) ccTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCt > 1:1685106/1‑46 (MQ=255) ccTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGc > 1:2609098/1‑70 (MQ=255) ccTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGc > 1:974548/1‑70 (MQ=255) ccTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt < 1:2565105/71‑1 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:3088128/49‑1 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:3097785/49‑1 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:59909/49‑1 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:2684046/49‑1 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:2202900/49‑1 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTa < 1:1639212/49‑1 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGa > 1:3911696/1‑51 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGa > 1:3916295/1‑51 (MQ=255) ttttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAcc > 1:735475/1‑71 (MQ=255) ttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAcccc > 1:3553450/1‑71 (MQ=255) ttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAcccc > 1:515417/1‑71 (MQ=255) ttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAcccc > 1:2196991/1‑71 (MQ=255) ttGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAccc > 1:672348/1‑70 (MQ=255) aaaTCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGAccgc > 1:3826733/1‑71 (MQ=255) aaaTCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGAccgc > 1:3263463/1‑71 (MQ=255) tCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt < 1:977187/51‑1 (MQ=255) tCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt < 1:3639733/51‑1 (MQ=255) tCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt < 1:2093553/51‑1 (MQ=255) | GTCAAATACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGC > W3110S.gb/2274180‑2274313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |