| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,291,258 | C→T | 22.1% | G326D (GGC→GAC) | yejO ← | predicted autotransporter outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,291,258 | 0 | C | T | 22.1% | 179.4 / 45.8 | 86 | G326D (GGC→GAC) | yejO | predicted autotransporter outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base T (14/5); total (44/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
ATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGA > W3110S.gb/2291191‑2291327 | atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAg > 1:1510954/1‑71 (MQ=255)atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg < 1:3836804/71‑1 (MQ=255)atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg < 1:688525/71‑1 (MQ=255)atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg > 1:1406722/1‑71 (MQ=255)atttatttGCGGCTTCATGACCTCAAGAATGACATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg > 1:390152/1‑71 (MQ=255) ggCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:873679/71‑1 (MQ=255) ttCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:1961444/68‑1 (MQ=255) ttCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:3647569/68‑1 (MQ=255) tCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAgc > 1:1166176/1‑70 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCgg > 1:425819/1‑69 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCgg > 1:240296/1‑69 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGc < 1:639565/70‑1 (MQ=255) ccTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGcc < 1:2692357/70‑1 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGcc > 1:1368258/1‑46 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttg > 1:597159/1‑71 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg > 1:3845873/1‑71 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg > 1:2723746/1‑71 (MQ=255) cAAGAATGCCATCATTCCCTCTGACAGAAACGTTAACCATTGTGcc > 1:2163773/1‑46 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3577867/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3556835/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:4054018/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttg < 1:866564/70‑1 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:1914550/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:2063970/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3119246/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:906101/69‑1 (MQ=255) aGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgtt < 1:3249729/71‑1 (MQ=255) aGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgtt < 1:978114/71‑1 (MQ=255) aGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgtt < 1:4002852/71‑1 (MQ=255) gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:3211482/71‑1 (MQ=255) gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:3405355/71‑1 (MQ=255) gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:466798/71‑1 (MQ=255) gAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:2175019/71‑1 (MQ=255) aTGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGa > 1:469088/1‑70 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGAt < 1:3506122/65‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGAt < 1:3930963/65‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAAc < 1:2578963/68‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCat < 1:2841277/71‑1 (MQ=255) catTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAt > 1:2387504/1‑57 (MQ=255) catTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAt > 1:3735726/1‑57 (MQ=255) catTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGAt < 1:2851752/64‑1 (MQ=255) catTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCata > 1:305417/1‑71 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:3344535/1‑59 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCatat > 1:1798640/1‑71 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCatat > 1:62889/1‑71 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCata > 1:1233237/1‑70 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCa > 1:4006200/1‑68 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCa > 1:3161638/1‑68 (MQ=255) atTCCCTCTGACTGAAACGTTAACAATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:107768/1‑59 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:1623475/1‑71 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:1795460/1‑71 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:494950/1‑71 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:2807425/1‑71 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCAATTGTTGATAACCATAtttt > 1:706179/1‑71 (MQ=255) ccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:2508411/1‑61 (MQ=255) ccTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:3306353/1‑61 (MQ=255) ctctGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:1560102/40‑1 (MQ=255) ctgactgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:1871888/1‑69 (MQ=255) ctgactgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:2161234/1‑69 (MQ=255) ctgactgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:2283477/1‑69 (MQ=255) ctgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:1375700/1‑69 (MQ=255) ctgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:4026478/1‑69 (MQ=255) tgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:772565/1‑68 (MQ=255) actgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:3031240/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:3027385/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1781568/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1309238/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGAt < 1:836628/71‑1 (MQ=255) ctgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATg < 1:669344/71‑1 (MQ=255) ctgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATg < 1:190365/71‑1 (MQ=255) tgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1704810/66‑1 (MQ=255) aCGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCttt > 1:1442150/1‑71 (MQ=255) cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAgaga < 1:2485672/63‑1 (MQ=255) cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAgaga < 1:3036438/63‑1 (MQ=255) cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGc > 1:799919/1‑67 (MQ=255) cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt < 1:224552/71‑1 (MQ=255) gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:2239529/71‑1 (MQ=255) gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:93666/71‑1 (MQ=255) gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:93096/71‑1 (MQ=255) gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:847583/71‑1 (MQ=255) ttAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:783461/38‑1 (MQ=255) ttAACCATTGTGCCAGACCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt > 1:1383695/1‑69 (MQ=255) aCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAgg < 1:3031230/70‑1 (MQ=255) ccATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAAc < 1:152542/43‑1 (MQ=255) gtgtCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:178682/1‑37 (MQ=255) gtgtCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt > 1:3011863/1‑71 (MQ=255) gtgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt > 1:2729456/1‑71 (MQ=255) gtgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGACGAATACGt > 1:3922380/1‑71 (MQ=255) tgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt < 1:3316919/70‑1 (MQ=255) tgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTT‑AGGAATACGTGa < 1:4084165/71‑1 (MQ=255) ccAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGAt < 1:3857783/50‑1 (MQ=255) ccAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGa < 1:1874439/70‑1 (MQ=255) ccAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGa < 1:2797666/70‑1 (MQ=255) | ATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGA > W3110S.gb/2291191‑2291327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |