| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,335,995 | C→T | 25.0% | pseudogene (3078/4605 nt) pseudogene (3078/4104 nt) |
yfaS ← yfaS ← |
ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,335,995 | 0 | C | T | 25.0% | 88.2 / 28.2 | 48 | pseudogene (3078/4605 nt) pseudogene (3078/4104 nt) | yfaS yfaS | ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base C (20/16); new base T (5/7); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01 | |||||||||||
TCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTG > W3110S.gb/2335927‑2336061 | tCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTAt > 1:1598101/1‑71 (MQ=255)tCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCAt > 1:3752155/1‑71 (MQ=255)tCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCAt > 1:229410/1‑71 (MQ=255) actactTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGa > 1:1430687/1‑71 (MQ=255) ctTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACAtt > 1:972634/1‑71 (MQ=255) cGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTctgctg < 1:1295057/71‑1 (MQ=255) atatTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTc < 1:2901054/71‑1 (MQ=255) atatTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTc < 1:593827/71‑1 (MQ=255) tatTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACAttt < 1:3833312/59‑1 (MQ=255) tatTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTc > 1:2063833/1‑70 (MQ=255) tatTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTc > 1:2430386/1‑70 (MQ=255) ttGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGa < 1:794466/52‑1 (MQ=255) ttGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGa < 1:2542273/52‑1 (MQ=255) ttGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTctg < 1:3690121/62‑1 (MQ=255) tGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACg > 1:791981/1‑71 (MQ=255) tGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACg > 1:726514/1‑71 (MQ=255) gCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTCTGCTGTTCAACgg > 1:1116048/1‑71 (MQ=255) gCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACg < 1:23175/70‑1 (MQ=255) ccTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTc < 1:2130548/61‑1 (MQ=255) cTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGc < 1:2983489/71‑1 (MQ=255) cGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTa > 1:2245693/1‑71 (MQ=255) cGGGCAACATCAGCGCATTATCGCCACCACCGAGTATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTa > 1:3483196/1‑71 (MQ=255) cATCAGCGCGTTATCGCCACCACCGAGTATGACAttt < 1:3229978/37‑1 (MQ=255) aTCAGCGCGTTATCGCCACCACCGAGCATGACAttttt > 1:2344433/1‑38 (MQ=255) aTCAGCGCGTTATCGCCACCACCGAGCATGACAttttt > 1:1169094/1‑38 (MQ=255) aTCAGCGCGTTATCGCCACCACCGAGCATGACAttttt > 1:3147187/1‑38 (MQ=255) aTCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACaa > 1:3761232/1‑70 (MQ=255) tCAGCGCGTTATCGCCACCACCGAGCATGACAttttt > 1:2876140/1‑37 (MQ=255) tCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACaaaa < 1:3468882/71‑1 (MQ=255) ttATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCa < 1:3790858/39‑1 (MQ=255) ttCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAg < 1:1935931/65‑1 (MQ=255) aTCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAAc > 1:3149744/1‑39 (MQ=255) aTCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGc > 1:1052053/1‑47 (MQ=255) aTCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTtg > 1:3491358/1‑71 (MQ=255) tCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGt < 1:2451716/49‑1 (MQ=255) cGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGt < 1:2287174/48‑1 (MQ=255) ccaccaCCGAGTATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCt < 1:164979/71‑1 (MQ=255) ccaccaCCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCt < 1:3068722/71‑1 (MQ=255) ccaccaCCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCt < 1:3905110/71‑1 (MQ=255) caccGAGTATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATg < 1:613010/71‑1 (MQ=255) caccGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTa > 1:1321526/1‑44 (MQ=255) caccGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTa > 1:1625893/1‑44 (MQ=255) caccGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTa > 1:1069084/1‑44 (MQ=255) caccGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATg < 1:3986540/71‑1 (MQ=255) caccGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATg < 1:280949/71‑1 (MQ=255) cGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTg < 1:3269685/71‑1 (MQ=255) aGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTtg > 1:642473/1‑58 (MQ=255) aGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTtg > 1:2087706/1‑58 (MQ=255) | TCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTG > W3110S.gb/2335927‑2336061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |