| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 86,762 | T→C | 13.9% | I378T (ATC→ACC) | ilvI → | acetolactate synthase III, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 86,762 | 0 | T | C | 13.9% | 85.2 / 7.7 | 36 | I378T (ATC→ACC) | ilvI | acetolactate synthase III, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (12/19); new base C (2/3); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01 | |||||||||||
GTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAGATGTT > W3110S.gb/86708‑86831 | gTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGActct > 1:4046372/1‑64 (MQ=255)gTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCg > 1:1436382/1‑70 (MQ=255) gCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGAc > 1:423324/1‑70 (MQ=255) ccTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCgg < 1:2888260/64‑1 (MQ=255) ccTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCgg < 1:2772698/64‑1 (MQ=255) ccTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGa < 1:1617017/71‑1 (MQ=255) tGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAg > 1:1785260/1‑71 (MQ=255) aaTATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGa < 1:441461/66‑1 (MQ=255) atatGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGa < 1:1842922/65‑1 (MQ=255) cacTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCgaga > 1:3075144/1‑41 (MQ=255) cTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCttt < 1:908759/45‑1 (MQ=255) cTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCttt < 1:3672985/45‑1 (MQ=255) cTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCttt < 1:2140592/45‑1 (MQ=255) cTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACg < 1:1145417/56‑1 (MQ=255) cTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACg < 1:2435597/56‑1 (MQ=255) tCACAGTGAAAAGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAGGgag < 1:2767330/62‑1 (MQ=255) cacaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGt > 1:494086/1‑71 (MQ=255) acaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGaa > 1:625328/1‑55 (MQ=255) acaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTg < 1:638028/71‑1 (MQ=255) aaaaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACg < 1:2206563/47‑1 (MQ=255) aaaaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACg < 1:1201091/47‑1 (MQ=255) aaaaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACg < 1:3922885/47‑1 (MQ=255) aaaaGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAg > 1:3407347/1‑50 (MQ=255) aaaGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAGGga < 1:2974506/52‑1 (MQ=255) aaaGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAGGga < 1:2225424/52‑1 (MQ=255) aaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGa > 1:3798596/1‑71 (MQ=255) aGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGAt < 1:1833925/71‑1 (MQ=255) aGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGAt < 1:1096095/71‑1 (MQ=255) gATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAgg > 1:2080824/1‑47 (MQ=255) ccGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCggg > 1:1820757/1‑69 (MQ=255) ggCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTa < 1:109538/48‑1 (MQ=255) cGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTa < 1:439218/49‑1 (MQ=255) gCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCg < 1:1872999/55‑1 (MQ=255) aTCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAGATGtt > 1:1431113/1‑71 (MQ=255) aTCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAGATGtt > 1:1245363/1‑71 (MQ=255) aTCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAGATGtt > 1:478219/1‑71 (MQ=255) | GTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAGATGTT > W3110S.gb/86708‑86831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |