| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,405,008 | C→T | 27.8% | E406E (GAG→GAA) | nuoF ← | NADH:ubiquinone oxidoreductase, chain F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,405,008 | 0 | C | T | 27.8% | 24.9 / 8.8 | 18 | E406E (GAG→GAA) | nuoF | NADH:ubiquinone oxidoreductase, chain F |
| Reads supporting (aligned to +/- strand): ref base C (4/9); new base T (2/3); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
TTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTG > W3110S.gb/2404940‑2405070 | ttGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTc > 1:2663740/1‑71 (MQ=255) ggAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCa > 1:1522857/2‑69 (MQ=255) aaCGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTg < 1:2578991/70‑1 (MQ=255) gCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCACTGCACCa < 1:2344309/71‑1 (MQ=255) tGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCACTGCACCAGGTGCg > 1:953536/1‑71 (MQ=255) cccGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAg > 1:3608200/1‑61 (MQ=255) ccTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTggg < 1:2165418/66‑1 (MQ=255) ccTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTggg < 1:1079277/66‑1 (MQ=255) cTCAAATTCTTCGAGGAAATATTTGATGGCGCTCTGTAACGGTTCCACTGCACCAGGTGCGTGGGCACAGa < 1:3431741/71‑1 (MQ=255) tCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTcc < 1:826407/45‑1 (MQ=255) tatTTGATGGCGCTCTGTAACGGCTCCACTGCAcc < 1:3518261/35‑1 (MQ=255) tatTTGATGGCGCTCTGTAACGGCTCCACTGCAcc < 1:2229342/35‑1 (MQ=255) ttGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGaa > 1:2344874/1‑71 (MQ=255) tGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGaa < 1:1446303/70‑1 (MQ=255) gcgcTCTGTAACGGCTCCACTGCACAAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGaca > 1:187479/1‑71 (MQ=255) cgcTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGacac < 1:1470316/71‑1 (MQ=255) ctctGTAACGGTTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGAcaca < 1:398412/70‑1 (MQ=255) tAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTg < 1:1702855/69‑1 (MQ=255) | TTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTG > W3110S.gb/2404940‑2405070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |