| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,413,837 | T→C | 37.5% | C277C (TGT→TGC) | yfbQ → | predicted aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,413,837 | 0 | T | C | 37.5% | 21.1 / 32.4 | 32 | C277C (TGT→TGC) | yfbQ | predicted aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (9/11); new base C (7/5); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
GAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTAT > W3110S.gb/2413768‑2413905 | gAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCtgtgtg > 1:303989/1‑71 (MQ=255)gAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCtgtgtg > 1:2942335/1‑71 (MQ=255) aCGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTCCt < 1:2914258/71‑1 (MQ=255) gAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCt < 1:834967/64‑1 (MQ=255) aaaCACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTgc < 1:3694425/71‑1 (MQ=255) acacGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTgcgc < 1:3959122/71‑1 (MQ=255) acacGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTgcgc < 1:356267/71‑1 (MQ=255) acacGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTgcgc < 1:442776/71‑1 (MQ=255) acGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCaa < 1:397523/71‑1 (MQ=255) gCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCAtt > 1:2000215/1‑71 (MQ=255) gCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCAtt > 1:1021530/1‑71 (MQ=255) gCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCAtt > 1:449561/1‑71 (MQ=255) gCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCAtt < 1:2624280/71‑1 (MQ=255) aCATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAg > 1:789490/1‑71 (MQ=255) aCATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAg > 1:223638/1‑71 (MQ=255) cATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCa < 1:2839301/69‑1 (MQ=255) cATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCa < 1:2597640/69‑1 (MQ=255) cATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAg < 1:1650614/70‑1 (MQ=255) cATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGa > 1:2745579/1‑71 (MQ=255) cATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGa > 1:2507046/1‑71 (MQ=255) tCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCaa < 1:2005972/56‑1 (MQ=255) cGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGAcc > 1:3583946/1‑70 (MQ=255) ggTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCt < 1:120449/71‑1 (MQ=255) tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCa > 1:1086308/1‑58 (MQ=255) tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCa > 1:3736359/1‑58 (MQ=255) tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCa > 1:3740830/1‑58 (MQ=255) tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAggt > 1:2189606/1‑71 (MQ=255) gAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAggt < 1:318705/69‑1 (MQ=255) gAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTc > 1:1964386/1‑37 (MQ=255) gAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTc > 1:2168824/1‑37 (MQ=255) cTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAggt > 1:2963320/1‑63 (MQ=255) cTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAg > 1:3355078/1‑61 (MQ=255) cAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCa < 1:585289/69‑1 (MQ=255) tGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAg < 1:554586/51‑1 (MQ=255) tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTAt > 1:3343903/1‑51 (MQ=255) tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTAt > 1:1233545/1‑51 (MQ=255) tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATttat > 1:737047/1‑71 (MQ=255) tgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAAtt < 1:3981384/66‑1 (MQ=255) | GAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTAT > W3110S.gb/2413768‑2413905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |