| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,426,403 | G→A | 29.7% | G113R (GGG→AGG) | yfcG → | predicted glutathione S‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,426,403 | 0 | G | A | 29.7% | 51.9 / 26.3 | 37 | G113R (GGG→AGG) | yfcG | predicted glutathione S‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base A (3/8); total (13/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01 | |||||||||||
CGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTT > W3110S.gb/2426340‑2426463 | cgTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCaaaa > 1:3838358/1‑71 (MQ=255) cgcgcCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAAtcatca < 1:3584794/71‑1 (MQ=255) acaTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGTGGCCGATGCTTGGGCAAAATCATCATTTTAATCa > 1:1422128/1‑71 (MQ=255) caTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat < 1:1212634/59‑1 (MQ=255) caTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat < 1:3678271/59‑1 (MQ=255) caTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat < 1:2147586/59‑1 (MQ=255) aTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:556879/71‑1 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:1662117/70‑1 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:2891338/70‑1 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:2186332/70‑1 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGc < 1:2775449/71‑1 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGc < 1:3813717/71‑1 (MQ=255) aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCAc > 1:936419/1‑67 (MQ=255) aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCa > 1:1552744/1‑66 (MQ=255) gTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:3469269/62‑1 (MQ=255) aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCa > 1:2100114/1‑61 (MQ=255) aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCa > 1:1761570/1‑61 (MQ=255) cTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCA‑TTTGATCACGCAGCCCCCCAAACCa < 1:2337333/70‑1 (MQ=255) aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:2289056/71‑1 (MQ=255) aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:803593/71‑1 (MQ=255) aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:4059040/71‑1 (MQ=255) aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:1732027/71‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCAtttt < 1:2080367/42‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCAtttt < 1:1753479/42‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCAtttt < 1:1998405/42‑1 (MQ=255) tAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc > 1:2463685/1‑71 (MQ=255) aGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg > 1:1185060/1‑47 (MQ=255) aGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc > 1:3760741/1‑70 (MQ=255) cGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTcc > 1:577548/1‑63 (MQ=255) ggACTGGGGCCGATGCTTGGGCAAAATCATCTTTTTAATCACGCAGCCCCCAAAACCATTCCTTACGCTAt > 1:3228164/1‑71 (MQ=255) ggACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTAt > 1:3845832/1‑71 (MQ=255) ggACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTAt > 1:1386256/1‑71 (MQ=255) cgCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGt < 1:3932028/70‑1 (MQ=255) gCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGtt < 1:1751547/71‑1 (MQ=255) gCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGtt < 1:3692163/71‑1 (MQ=255) gCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGtt < 1:502717/71‑1 (MQ=255) gCCGATGCTTAGGCAAAATCATGATTTTAATCACGCAGCCCACCAAACCATTCCTTACGCTATTGAACGtt < 1:3972325/71‑1 (MQ=255) | CGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTT > W3110S.gb/2426340‑2426463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |