| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,449,567 | C→T | 23.1% | V17I (GTT→ATT) | yfcL ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,449,567 | 0 | C | T | 23.1% | 156.7 / 44.5 | 78 | V17I (GTT→ATT) | yfcL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (23/37); new base T (9/9); total (32/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
ATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTCATTGTAA > W3110S.gb/2449500‑2449633 | aTGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCAt < 1:2104569/71‑1 (MQ=255)aTGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCAt < 1:2441643/71‑1 (MQ=255) tGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATAcc < 1:2400392/66‑1 (MQ=255) tGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATAcc < 1:3449853/66‑1 (MQ=255) tGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATAcc < 1:1463220/66‑1 (MQ=255) tGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTc < 1:3874017/69‑1 (MQ=255) gTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAt > 1:1156047/1‑71 (MQ=255) gTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAt > 1:3473154/1‑71 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACACCTCATCATCACTGGCATGGTCAATCATACCg > 1:3562241/1‑65 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAATCATACCGTCGAtt < 1:3632846/71‑1 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAATCATACCGTCGAt > 1:757967/1‑70 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAATCATACCGTCGAt > 1:1292720/1‑70 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGt > 1:3199190/1‑66 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGt > 1:2003928/1‑66 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGt > 1:2186184/1‑66 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:3182315/71‑1 (MQ=255) tCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:2259875/71‑1 (MQ=255) ccACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAg < 1:54153/71‑1 (MQ=255) cACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAg < 1:1967946/70‑1 (MQ=255) cAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGa > 1:2644297/1‑56 (MQ=255) aaTACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGt > 1:3314947/1‑71 (MQ=255) aaTACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:536118/56‑1 (MQ=255) aaTACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:327214/56‑1 (MQ=255) aaTACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:598190/56‑1 (MQ=255) aaTACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:952509/56‑1 (MQ=255) aaTACCCACTGGCAAACAACTAATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:1454330/56‑1 (MQ=255) cACTGGCAAACAACTCATCATCACTGGCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTGATTCa < 1:2975251/71‑1 (MQ=255) cACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTc > 1:772818/1‑70 (MQ=255) cACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCa < 1:3422957/71‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAATCATACCGTCGATTAATGCCAg < 1:1977765/56‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAATCATACCGTCGATTAATGCCAg < 1:631052/56‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCAt < 1:373707/38‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCAt < 1:1631646/38‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAg < 1:3534083/56‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAg < 1:3406368/56‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAg < 1:1358003/56‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAg < 1:3238229/56‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAg < 1:375469/56‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCaaa < 1:2266862/71‑1 (MQ=255) cTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCa < 1:873226/69‑1 (MQ=255) ggCAAACAACTCATCATCACTGGCATGGTCAACCATAc > 1:688835/1‑38 (MQ=255) ggCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCg > 1:3836358/1‑60 (MQ=255) gCAAACAACTCATCATCACTGGCATGGTCAACCATAcc > 1:3525117/1‑38 (MQ=255) gCAAACAACTCATCATCACTGGCATGGTCAACCATAcc > 1:3080784/1‑38 (MQ=255) gCAAACAACTCATCATCACTGGCATGGTCAACCATAcc > 1:1566287/1‑38 (MQ=255) gCAAACAACTCATCATCACTGGCATGGTCAACCATAcc > 1:1373018/1‑38 (MQ=255) gCAAACAACTCATCATCACTGGCATGGTCAACCATAcc > 1:3878082/1‑38 (MQ=255) aacaacTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:48744/42‑1 (MQ=255) aacaacTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:1212272/42‑1 (MQ=255) aacaacTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:2577812/42‑1 (MQ=255) aacaacTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:3050013/42‑1 (MQ=255) acaacTCATCATCACTGGCATGGTCAACCATACCGTCGAtt < 1:2458625/41‑1 (MQ=255) caacTCATCATCACTGGCATGGTCAATCATACCGTCGATTAATGCCAGaa > 1:3795882/1‑50 (MQ=255) caacTCATCATCACTGGCATGGTCAACCATACCGTCGATTaa > 1:3879846/1‑42 (MQ=255) aaacTCATCATCACTGGCATGGTCAATCATACCGTCGATTAATGCCa > 1:3111982/2‑47 (MQ=255) acTCATCATCACTGGCATGGT‑AACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGatca < 1:2966105/71‑1 (MQ=255) cTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGatca < 1:3341098/71‑1 (MQ=255) catcatcaCTGGCATGGTCAACCATACCGTCGATTAATGACAGaa > 1:907842/1‑45 (MQ=255) tcatcaCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCAtctt < 1:2809301/71‑1 (MQ=255) atcaCTGGCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCAtcttct > 1:2212705/1‑71 (MQ=255) atcaCTGGCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTGAATCAAACTCCGCGATCAtcttct > 1:2581990/1‑71 (MQ=255) tcaCTGGCATGGTCAATCATACCGTCGATTAATGCCAGAATGCg < 1:3363974/44‑1 (MQ=255) tcaCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCa < 1:155474/51‑1 (MQ=255) tcaCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATcttctt < 1:3875841/71‑1 (MQ=255) caCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGatca > 1:2407288/1‑63 (MQ=255) caCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGatca > 1:2619885/1‑63 (MQ=255) aCTGGCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTg > 1:136783/1‑44 (MQ=255) aCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTc > 1:2848910/1‑71 (MQ=255) aCTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTc > 1:948837/1‑71 (MQ=255) gCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTc < 1:1847349/70‑1 (MQ=255) gCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTc < 1:1598831/70‑1 (MQ=255) gCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTc < 1:2753917/70‑1 (MQ=255) gCATGGTCAATCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTc < 1:3634420/70‑1 (MQ=255) gCATGGT‑AACCATACCGTCGATTAATGCCAGAATGCg > 1:1659075/1‑37 (MQ=38) gTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTCATTGt > 1:2310732/1‑70 (MQ=255) gTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTCATTGt > 1:1681920/1‑70 (MQ=255) gTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTCATTGTa < 1:3296275/71‑1 (MQ=255) tCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTCATTGTaa < 1:1864855/71‑1 (MQ=255) ccATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCttt < 1:3497755/56‑1 (MQ=255) ccATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCttt < 1:3576391/56‑1 (MQ=255) | ATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACCGTCGATTAATGCCAGAATGCGTGATTCAAACTCCGCGATCATCTTCTTTCCTCATTGTAA > W3110S.gb/2449500‑2449633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |