| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,457,604 | A→G | 8.7% | L60P (CTG→CCG) | yfcS ← | predicted periplasmic pilus chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,457,604 | 0 | A | G | 8.7% | 185.3 / 8.9 | 69 | L60P (CTG→CCG) | yfcS | predicted periplasmic pilus chaperone |
| Reads supporting (aligned to +/- strand): ref base A (28/35); new base G (4/2); total (32/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CACTGGCGGCAGCACAGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCA > W3110S.gb/2457534‑2457673 | cACTGGCGGCAGCACAGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCa < 1:2123516/71‑1 (MQ=255)cACTGGCGGCAGCACAGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCa < 1:1474247/71‑1 (MQ=255) gccagcaCAGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAgg < 1:238563/69‑1 (MQ=255) gcagcaCAGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAggg < 1:1719883/71‑1 (MQ=255) acaGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGa > 1:3991330/1‑67 (MQ=255) acaGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGttt > 1:2442708/1‑71 (MQ=255) acaGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGttt > 1:1682115/1‑71 (MQ=255) caGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCGGATAAGGGAGTTTc > 1:382590/1‑71 (MQ=255) caGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTc > 1:2544145/1‑71 (MQ=255) caGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCAAGCTTTGAGCCAGATAAGGGAGTTTc > 1:3520465/1‑71 (MQ=255) aGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATaa > 1:2684230/1‑61 (MQ=255) aGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCg < 1:2605059/71‑1 (MQ=255) gTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCAAGCTTTGAGCCAGATAAGGGAGTTTCg > 1:104816/1‑70 (MQ=255) aaCGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCGGATAAGGGAGTTTCGGATc > 1:3595520/1‑71 (MQ=255) cGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGt < 1:1178704/71‑1 (MQ=255) cGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATa < 1:1214452/51‑1 (MQ=255) cGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGttat < 1:3982399/71‑1 (MQ=255) cGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGttat < 1:2451686/71‑1 (MQ=255) cGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGttat < 1:3464235/71‑1 (MQ=255) gTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATa < 1:765709/47‑1 (MQ=255) gATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAg > 1:3598079/1‑47 (MQ=255) gATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAg > 1:3552915/1‑47 (MQ=255) aTCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAg > 1:886804/1‑71 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCGGATAAGGGAGTTTCGGATCGTTATTACGCAGCg > 1:3756101/1‑71 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCGGATAAGGGAGTTTCGGATCGTTATTACGCAGCg > 1:1533951/1‑71 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAg > 1:3381108/1‑39 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAg > 1:2376523/1‑48 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGc > 1:1208754/1‑70 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCg > 1:1720602/1‑71 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCg > 1:73355/1‑71 (MQ=255) cTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCg > 1:2547180/1‑71 (MQ=255) gTTACCCTTTTCATCTTCAATCCAGCTTTGAGGCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGt < 1:2527957/69‑1 (MQ=255) gTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGt < 1:1479049/69‑1 (MQ=255) gTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGt < 1:1102476/69‑1 (MQ=255) gTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGt < 1:2117792/69‑1 (MQ=255) gTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGt < 1:1653838/69‑1 (MQ=255) gTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGt < 1:3903506/69‑1 (MQ=255) ttACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCAc > 1:10247/1‑71 (MQ=255) aCCCTTTTCATCTTCTATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGt < 1:2879273/53‑1 (MQ=255) ccTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGTGTTTCGGATCGTTATTACGCAGCGTCACGCtg < 1:3759680/71‑1 (MQ=255) ccTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCt < 1:3707069/70‑1 (MQ=255) ttttCATCTTCAATCCATCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCt < 1:2583691/68‑1 (MQ=255) ttttCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGttat < 1:528936/52‑1 (MQ=255) ttttCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGttat < 1:3463114/52‑1 (MQ=255) ttttCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCt < 1:3730384/68‑1 (MQ=255) tttCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGt < 1:3362797/48‑1 (MQ=255) ttCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCtgatt > 1:3455661/1‑70 (MQ=255) tCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGtta > 1:2256227/1‑48 (MQ=255) tCTTCAATCCAGCTTTGAGCCGGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCt < 1:824521/62‑1 (MQ=255) tCTTCAATCCAGCTTTGAGCCGGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCt < 1:2415878/62‑1 (MQ=255) tCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCAc < 1:2432808/59‑1 (MQ=255) cTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTg > 1:1008629/1‑71 (MQ=255) ttCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGt < 1:336629/41‑1 (MQ=255) ttCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGt < 1:274629/41‑1 (MQ=255) ttCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCtgatt < 1:3225498/64‑1 (MQ=255) ttCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCtgatt < 1:3205487/64‑1 (MQ=255) ttCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCtgatt < 1:3970295/64‑1 (MQ=255) ttCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTgattgatt > 1:263500/1‑68 (MQ=255) ttCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGt > 1:1378080/1‑71 (MQ=255) cAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGAttt < 1:374539/67‑1 (MQ=255) cAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTc < 1:1760602/71‑1 (MQ=255) cAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTc < 1:1210191/71‑1 (MQ=255) gccttGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCAt > 1:1203376/4‑71 (MQ=255) gCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCAt > 1:3172550/1‑71 (MQ=255) gCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCAt > 1:1567158/1‑71 (MQ=255) ttGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGc > 1:1475658/1‑47 (MQ=255) ttGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCAt > 1:1486187/1‑68 (MQ=255) ttGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTa > 1:201910/1‑70 (MQ=255) cAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCa < 1:3221829/71‑1 (MQ=255) cAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCa < 1:271423/71‑1 (MQ=255) cAGATAAGGGAGTTTCCGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCa < 1:1643040/71‑1 (MQ=255) | CACTGGCGGCAGCACAGTTAACGGCGAGGTGATCTTGTTACCCTTTTCATCTTCAATCCAGCTTTGAGCCAGATAAGGGAGTTTCGGATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCA > W3110S.gb/2457534‑2457673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |