| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,461,423 | G→A | 22.2% | intergenic (‑331/+350) | yfcV ← / ← sixA | predicted fimbrial‑like adhesin protein/phosphohistidine phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,461,423 | 0 | G | A | 22.2% | 207.0 / 54.9 | 99 | intergenic (‑331/+350) | yfcV/sixA | predicted fimbrial‑like adhesin protein/phosphohistidine phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (39/38); new base A (13/9); total (52/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
ATATATTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAACACTATAG > W3110S.gb/2461359‑2461482 | atatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACata > 1:957644/1‑71 (MQ=255)atatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACata > 1:3519706/1‑71 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat < 1:750372/71‑1 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat < 1:2825202/71‑1 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACata > 1:4047681/1‑70 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACata > 1:2998601/1‑70 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACata > 1:1777555/1‑70 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACa < 1:1021283/68‑1 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACatat < 1:3891808/71‑1 (MQ=255) tatatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACata > 1:3821320/1‑70 (MQ=255) atatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat > 1:1054774/1‑70 (MQ=255) atatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat > 1:827495/1‑70 (MQ=255) tatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATaa < 1:1654266/71‑1 (MQ=255) tatTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACata > 1:2827326/1‑68 (MQ=255) tAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACata > 1:1550427/1‑64 (MQ=255) tAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGc < 1:279145/70‑1 (MQ=255) tAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGc < 1:3377901/70‑1 (MQ=255) tAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGc > 1:2024855/1‑70 (MQ=255) tAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGc > 1:3029002/1‑70 (MQ=255) gAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGAt < 1:1702323/71‑1 (MQ=255) gAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGAt < 1:3477004/71‑1 (MQ=255) gAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGAt < 1:336827/71‑1 (MQ=255) gAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGAt < 1:57346/71‑1 (MQ=255) aaaaCAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTgg < 1:2512000/71‑1 (MQ=255) ggAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTaa < 1:659513/71‑1 (MQ=255) ggAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTaa < 1:2992722/71‑1 (MQ=255) ggAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTaa < 1:293254/71‑1 (MQ=255) aGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAAccc > 1:1400414/1‑71 (MQ=255) tatCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCg < 1:3221651/53‑1 (MQ=255) tatCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCg < 1:2506250/53‑1 (MQ=255) tatCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCg < 1:373559/53‑1 (MQ=255) tatCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCg < 1:620907/53‑1 (MQ=255) tatCAACAATGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCtt > 1:1546868/1‑71 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATg > 1:422971/1‑38 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat < 1:385184/45‑1 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat < 1:2462473/45‑1 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat < 1:2827000/45‑1 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat < 1:3195070/45‑1 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACatat < 1:375137/45‑1 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACAtata > 1:2484601/1‑46 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACAtata > 1:1757857/1‑46 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACAtata > 1:3214604/1‑46 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACAtata > 1:1856834/1‑46 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACAtata > 1:1306152/1‑46 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACAtata > 1:1906684/1‑46 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGa > 1:2301358/1‑52 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTACCCCGCtttt > 1:1142140/1‑71 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCtttt > 1:2385438/1‑71 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCtttt > 1:115929/1‑71 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCtttt > 1:1869104/1‑71 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCtttt > 1:2360561/1‑71 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCtttt > 1:619342/1‑71 (MQ=255) tCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCttt < 1:1993512/70‑1 (MQ=255) aacaaTGAATGCCAATAGCTTAAAAAACAAACAATGAACATATaa > 1:41417/1‑45 (MQ=255) aacaaTGAATGCCAATAGCTTAAAAAACAAACAATGAACATATaa > 1:2618653/1‑45 (MQ=255) aacaaTGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCa > 1:611922/1‑56 (MQ=255) aacaaTGAATGCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCa > 1:513852/1‑56 (MQ=255) acaaTGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTc < 1:3248537/71‑1 (MQ=255) aatgaatgCCAATAGCTTAAAAAACAAACAATGAACatat < 1:1800553/40‑1 (MQ=255) atgaatgCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGa > 1:1338251/1‑70 (MQ=255) tgaatgCCAATAGCTTAAAAAACAAACAATGAACATATaa > 1:3002109/1‑40 (MQ=255) tgaatgCCAATAGCTTAAAAAACAAACAATGAACATATaa > 1:1093335/1‑40 (MQ=255) tgaatgCCAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGata > 1:2516213/1‑71 (MQ=255) gaatgCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGata > 1:3502504/1‑70 (MQ=255) atgCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGatataa < 1:1791193/71‑1 (MQ=255) tgCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGatataat < 1:2932389/71‑1 (MQ=255) cAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGa < 1:3376562/62‑1 (MQ=255) cAATAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGa < 1:3945612/62‑1 (MQ=255) aaTAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGatat < 1:1863302/64‑1 (MQ=255) aaTAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGatat < 1:2725873/64‑1 (MQ=255) aTAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata < 1:3386765/71‑1 (MQ=255) aTAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata < 1:1471144/71‑1 (MQ=255) aTAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata < 1:1310011/71‑1 (MQ=255) aTAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCa > 1:780176/1‑42 (MQ=255) aTAGCTTAAAAAACAAACAATAAACATATAATGCGATTGGCa > 1:920003/1‑42 (MQ=255) tAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata > 1:802953/1‑70 (MQ=255) tAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata > 1:1025563/1‑70 (MQ=255) tAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata < 1:3527258/70‑1 (MQ=255) tAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata > 1:1765402/1‑70 (MQ=255) tAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata > 1:204304/1‑70 (MQ=255) aGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATaaa > 1:4017309/1‑71 (MQ=255) aGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATaaa > 1:2330813/1‑71 (MQ=255) gCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTc > 1:3106865/1‑55 (MQ=255) gCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATaaa > 1:646447/1‑70 (MQ=255) gCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAac < 1:173263/71‑1 (MQ=255) gCTTAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAac < 1:92607/71‑1 (MQ=255) tAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAacac > 1:1210922/1‑70 (MQ=255) tAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAacac > 1:4024414/1‑70 (MQ=255) tAAAAAACAAACAATAAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAacac > 1:1159677/1‑70 (MQ=255) aaaaaaCAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAacac < 1:1613706/69‑1 (MQ=255) aaaaaaCAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAACACta > 1:2178872/1‑71 (MQ=255) aaaaaCAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAACACta < 1:2435189/70‑1 (MQ=255) aaaaaCAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAACACta < 1:1123582/70‑1 (MQ=255) aaacaaacAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAACACTATAg > 1:3368535/1‑71 (MQ=255) caaTGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGatataatata > 1:3450261/1‑52 (MQ=255) aaTGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata < 1:1902387/53‑1 (MQ=255) aaTGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata < 1:1658347/53‑1 (MQ=255) aTGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAAtatata < 1:4022185/52‑1 (MQ=255) tGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGa < 1:477742/40‑1 (MQ=255) tGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGa < 1:3584907/40‑1 (MQ=255) | ATATATTTAGAAAAAACAGGAAGCATATCAACAATGAATGCCAATAGCTTAAAAAACAAACAATGAACATATAATGCGATTGGCATTAACCCGCTTTTGTCGATATAATATATAAACACTATAG > W3110S.gb/2461359‑2461482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |