| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,472,806 | C→T | 32.1% | D272D (GAC→GAT) | intS → | predicted prophage CPS‑53 integrase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,472,806 | 0 | C | T | 32.1% | 67.2 / 43.4 | 53 | D272D (GAC→GAT) | intS | predicted prophage CPS‑53 integrase |
| Reads supporting (aligned to +/- strand): ref base C (9/27); new base T (7/10); total (16/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
AAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTT > W3110S.gb/2472737‑2472868 | aaaTAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGAcc < 1:2566454/71‑1 (MQ=255) aaTAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCa < 1:2885800/71‑1 (MQ=255) aaTAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCa < 1:2997447/71‑1 (MQ=255) aTAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCa < 1:1806137/70‑1 (MQ=255) tAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAgg > 1:1137663/1‑71 (MQ=255) ggATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGt < 1:2557301/70‑1 (MQ=255) ggATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAgg > 1:1481419/1‑69 (MQ=255) aTCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTg < 1:3007267/70‑1 (MQ=255) aTCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTg < 1:2139918/70‑1 (MQ=255) tCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGTTTCCTATGTCAGACCAGGTAGTTg < 1:2692453/69‑1 (MQ=255) tCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTg < 1:2785726/69‑1 (MQ=255) tCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGaa < 1:3778865/71‑1 (MQ=255) tCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGaa < 1:1176660/71‑1 (MQ=255) tCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGa < 1:2848447/70‑1 (MQ=255) cATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTGAACTtc > 1:573103/1‑71 (MQ=255) cATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTGAACTtc > 1:1666432/1‑71 (MQ=255) aTCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTGAACTtct < 1:410467/71‑1 (MQ=255) aTCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTtct < 1:1002339/71‑1 (MQ=255) tctaCGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAAc < 1:530688/63‑1 (MQ=255) tCGACGCCAGTTTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCa < 1:806874/55‑1 (MQ=255) tCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTGAACtt < 1:1359632/68‑1 (MQ=255) tCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTGAACtt < 1:1668640/68‑1 (MQ=255) tCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAgg < 1:1771446/57‑1 (MQ=255) tCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAgg < 1:3120568/57‑1 (MQ=255) tCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAgg < 1:2643583/57‑1 (MQ=255) tCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAAc < 1:1508289/66‑1 (MQ=255) cGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAgg < 1:1255360/56‑1 (MQ=255) gACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAg > 1:3648230/1‑58 (MQ=255) gACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTGAACTtct > 1:1143749/1‑68 (MQ=255) gACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCAc < 1:1516916/71‑1 (MQ=255) gACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCAc < 1:1496742/71‑1 (MQ=255) cAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGc > 1:3206084/1‑71 (MQ=255) cAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGc > 1:1390398/1‑71 (MQ=255) gtgATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAg > 1:3627158/1‑45 (MQ=255) gtgATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAg > 1:3870644/1‑45 (MQ=255) atgaAATGACGCAAAATTCATGTGGTTCCTATGTCAGACCAg > 1:688075/1‑42 (MQ=255) tgaAAGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTGAACTtct < 1:143979/55‑1 (MQ=255) aaGGACGCAAAATTCATGTGGTTCCTATGTCAGATCAGGTAGTTg < 1:1758985/45‑1 (MQ=255) aaaaTTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAAcc < 1:801636/71‑1 (MQ=255) aaaaTTCATGTGGTTCATATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAAcc < 1:1728478/71‑1 (MQ=255) aaTTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCaaagc < 1:3045271/69‑3 (MQ=255) aTTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTa < 1:1421856/51‑1 (MQ=255) ttCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTa < 1:1995610/50‑1 (MQ=255) aTGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCa < 1:2985130/71‑1 (MQ=255) tgGTTCCTATGTCAGATCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCaga < 1:3698524/70‑1 (MQ=255) gTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCagaggc > 1:1141504/1‑69 (MQ=255) gTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCagaggc > 1:2398231/1‑69 (MQ=255) ccTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAgtttgt > 1:3749273/1‑71 (MQ=255) tGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGtttt < 1:613589/70‑1 (MQ=255) tGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGtttt < 1:3299068/70‑1 (MQ=255) gTCAGATCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCa > 1:3528381/1‑41 (MQ=255) tCAGATCAGGTAGTTGAACTTCTCACTACGCTAAGCTc < 1:1400207/38‑1 (MQ=255) tCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAgtttgtt < 1:1722196/66‑1 (MQ=255) aGATCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCaaa > 1:2380253/1‑46 (MQ=255) | AAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTT > W3110S.gb/2472737‑2472868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |