| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,497,222 | A→G | 25.0% | Y59H (TAT→CAT) | oxc ← | predicted oxalyl‑CoA decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,497,222 | 0 | A | G | 25.0% | 60.8 / 20.8 | 36 | Y59H (TAT→CAT) | oxc | predicted oxalyl‑CoA decarboxylase |
| Reads supporting (aligned to +/- strand): ref base A (15/12); new base G (5/4); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCTGGCGCAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGAATGCCTTCCGCCTGGGCATG > W3110S.gb/2497153‑2497279 | aTCCTGGCGCAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAg < 1:602282/71‑1 (MQ=255)aTCCTGGCGCAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAg < 1:2213929/71‑1 (MQ=255) cTGGCGCAGAAACTGTCAGGCAGATCCCTGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATGGCCt > 1:501858/1‑71 (MQ=255) cTGGCGCAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATGGCCt > 1:493688/1‑71 (MQ=255) ggCGCAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCCTGGCCTGc > 1:3934257/1‑71 (MQ=255) ggCGCAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGc > 1:337815/1‑71 (MQ=255) cgcAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGcc < 1:3129191/70‑1 (MQ=255) cAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGcc < 1:118620/68‑1 (MQ=255) aaCTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCt > 1:3572201/1‑71 (MQ=255) aaCTGTCAGGCAGATCCACGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCt > 1:2322376/1‑71 (MQ=255) aGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTAATGAc > 1:691113/1‑70 (MQ=255) aGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGaaaa > 1:1326067/1‑71 (MQ=255) gATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGaa < 1:1459465/68‑1 (MQ=255) gATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGaa < 1:843001/68‑1 (MQ=255) aTCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATGGCCTGCCGACTGCTCATGACGAAAAcc < 1:94846/71‑1 (MQ=255) ccccGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGTCATAGCCTGCCGACTGCTCATGACGAAAACCaa > 1:1599185/1‑71 (MQ=255) ccccGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCaa > 1:632622/1‑71 (MQ=255) ccccGGTTTTTGGGTAAGAAAACCGCTCGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCaa > 1:3376149/1‑71 (MQ=255) tttGGGTAAGAAAACCGCTTGCCGCAGCGGCATGGCCTGCCGACTGCTCATGACGAAAACCAATATAACg > 1:1899978/1‑70 (MQ=255) tttGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACg < 1:1209434/70‑1 (MQ=255) gTAAGAAAACCGCTTGCCGCAGCGGCATGGCCTGCCGACTGCTCATGACGAAAACCAatat < 1:1867255/61‑1 (MQ=255) gTAAGAAAACCGCTTGCCGCAGCGGCATGGCCTGCCGACTGCTCATGACGAAAACCAATATAACGaa < 1:737382/67‑1 (MQ=255) aaaaCCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATg > 1:2165976/1‑42 (MQ=255) aaaaCCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGaa > 1:636419/1‑62 (MQ=255) aaaaCCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGaa > 1:2164264/1‑62 (MQ=255) aaaaCCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGaa > 1:1890040/1‑62 (MQ=255) aaaaCCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGAATGCCTTcc < 1:2340301/70‑1 (MQ=255) aaaCCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCa > 1:693225/1‑39 (MQ=255) aaCCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGAATGCCTTCCGcc < 1:2768673/71‑1 (MQ=255) gCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGAATGCCTTCCGCCTggg < 1:3612274/71‑1 (MQ=255) gCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGAATGCCTTCCGCCTGGGCATg > 1:2534259/1‑71 (MQ=255) gCAGCGGCATGGCCTGCCGACTGCTCATGACGAAAACCAATATAACg < 1:296357/47‑1 (MQ=255) aGCGGCATGGCCTGCCGACTGCTCATGACGAAAACCAATATAACGaa > 1:888618/1‑47 (MQ=255) gCGGCATAGCCTGCCGACTGCTCATGACGAAAAcc < 1:2134802/35‑1 (MQ=255) aTAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGAATGCCTTCCGCCt > 1:3711002/1‑53 (MQ=255) tAGCCTGCCGACTGCTCATGACGAAAACCAATATAACg < 1:3015251/38‑1 (MQ=255) | ATCCTGGCGCAGAAACTGTCAGGCAGATCCCCGGTTTTTGGGTAAGAAAACCGCTTGCCGCAGCGGCATAGCCTGCCGACTGCTCATGACGAAAACCAATATAACGAATGCCTTCCGCCTGGGCATG > W3110S.gb/2497153‑2497279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |