| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,508,088 | C→A | 30.1% | G615V (GGC→GTC) | ypdD ← | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,508,088 | 0 | C | A | 30.1% | 115.3 / 27.1 | 56 | G615V (GGC→GTC) | ypdD | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Reads supporting (aligned to +/- strand): ref base C (27/12); new base A (9/8); total (36/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACAT > W3110S.gb/2508024‑2508155 | gCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGtt < 1:2918506/71‑1 (MQ=255) ctcatactcaGCTCATCAAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCAc > 1:3683969/1‑70 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGTCAGATAACGGCTTTCACCGCCCAGTTCACCg > 1:2361267/1‑70 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCg > 1:3658990/1‑70 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCg > 1:2878805/1‑70 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCg > 1:1410778/1‑70 (MQ=255) atactcaGCTCATCCAGGCCCCGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCg > 1:4022678/1‑69 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa < 1:2246787/71‑1 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGc > 1:1391905/1‑70 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCa < 1:2759365/71‑1 (MQ=255) atacacaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGCCAGATAACGGCTTTCACCGCCCAGTACACCGc > 1:4027492/1‑70 (MQ=255) ctcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCAAAt > 1:417976/1‑71 (MQ=255) tCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAAt < 1:402606/65‑1 (MQ=255) tCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAAt < 1:2144989/65‑1 (MQ=255) tCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAAt < 1:1301885/65‑1 (MQ=255) tCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTAc > 1:746835/1‑71 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGc > 1:1989443/1‑65 (MQ=255) aTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCAAATGc > 1:2792414/1‑65 (MQ=255) aTCCAGGCCCAGCCCAAGAAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACAGCAAATGc > 1:2139302/1‑65 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCa < 1:2214410/71‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGct < 1:383877/59‑2 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCa < 1:1733523/59‑1 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCa < 1:728876/59‑1 (MQ=255) ccAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCAAATGCCTa > 1:2562335/1‑66 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGc > 1:3195710/1‑44 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGc > 1:936881/1‑44 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGc > 1:4023420/1‑44 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGt > 1:1929665/1‑49 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa > 1:371867/1‑57 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCAtt > 1:1227555/1‑71 (MQ=255) cagcgcaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa > 1:3330839/1‑51 (MQ=255) cagcccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGc > 1:134982/1‑50 (MQ=255) cagccaaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCa > 1:2346118/1‑51 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2100628/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:3729279/71‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2201267/71‑1 (MQ=255) cAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATgcg < 1:393220/71‑1 (MQ=255) gTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATgcg > 1:1894357/1‑69 (MQ=255) aGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGt > 1:2531135/1‑71 (MQ=255) gCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTg > 1:3200407/1‑71 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTAc > 1:663519/1‑41 (MQ=255) aGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAAc > 1:2276625/1‑71 (MQ=255) gATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCt < 1:353486/38‑1 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTa < 1:3437265/71‑1 (MQ=255) tAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTaa > 1:1673255/1‑67 (MQ=255) tAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTaa > 1:132011/1‑67 (MQ=255) aaCGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATgcgc > 1:2145587/1‑58 (MQ=255) aaCGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAg > 1:717102/1‑60 (MQ=255) cGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2285095/49‑1 (MQ=255) ggCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:849256/48‑1 (MQ=255) tttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGt > 1:3830544/1‑55 (MQ=255) tttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGt > 1:2185222/1‑55 (MQ=255) tttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGt > 1:2692938/1‑55 (MQ=255) tttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgctg > 1:4014023/1‑70 (MQ=255) tttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgctg > 1:218941/1‑70 (MQ=255) tttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgctg > 1:129841/1‑70 (MQ=255) cACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:2371754/70‑1 (MQ=255) aCCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACa > 1:791426/1‑71 (MQ=255) ccGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACAt < 1:3870632/71‑1 (MQ=255) | GCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACAT > W3110S.gb/2508024‑2508155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |