| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,521,463 | C→T | 48.5% | A606T (GCG→ACG) | yfeA ← | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,521,463 | 0 | C | T | 48.5% | ‑1.5 / 45.6 | 33 | A606T (GCG→ACG) | yfeA | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (6/11); new base T (3/13); total (9/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GCTCGATGTTGTACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGATAACGCTTAAACAGACGAATAATCCGCCCGGCAATATTCTTTTGCAGCAGCGTG > W3110S.gb/2521394‑2521533 | gctCGATGTTGTACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGcc > 1:510129/1‑71 (MQ=255)gctCGATGTTGTACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGc > 1:578187/1‑70 (MQ=255) ttgtACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGc > 1:3923622/1‑62 (MQ=255) ttgtACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:840914/71‑1 (MQ=255) ttgtACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:836160/71‑1 (MQ=255) gtACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCggg > 1:1228819/1‑67 (MQ=255) gtACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGgag < 1:161311/70‑1 (MQ=255) gtACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGgag < 1:3208503/70‑1 (MQ=255) gtACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCg > 1:1677279/1‑65 (MQ=255) cATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGAtgt < 1:3741453/71‑1 (MQ=255) cATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCg < 1:2459688/62‑1 (MQ=255) tgctgcTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGa > 1:3221569/1‑71 (MQ=255) ctgctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGAt < 1:1439412/70‑1 (MQ=255) ctgctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGAt < 1:3930368/70‑1 (MQ=255) ctTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCggg > 1:2430107/1‑57 (MQ=255) tCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGATaa > 1:3703159/1‑66 (MQ=255) cGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGGAGATGTGATAACGCTTa < 1:3152486/68‑1 (MQ=255) gTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGATAACGCTTAAACa < 1:2573411/71‑1 (MQ=255) aaaaCGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGga < 1:47794/46‑1 (MQ=255) aaaaCGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGAtgt < 1:2788649/51‑1 (MQ=255) ccTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:3489356/40‑1 (MQ=255) ccTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:247148/40‑1 (MQ=255) ccTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:3971047/40‑1 (MQ=255) ccTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:4056037/40‑1 (MQ=255) ccTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:1883936/40‑1 (MQ=255) ccTGCTCCTCGGTGATCTCAAGAATGACCGTCTGCGGGga < 1:3187701/40‑1 (MQ=255) ggTGATCTCAAGAATGACCGTCTGCGGGGAGATGTGATAACGCTTAAACAGACGAATAATCCGCCCGGc < 1:819964/69‑1 (MQ=255) ggTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGATAACGCtt > 1:2047588/1‑45 (MQ=255) ggTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGATAACGCTTa < 1:3540731/46‑1 (MQ=255) ggTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGATAACGCTTa < 1:965924/46‑1 (MQ=255) aTCTCAAGAATGACCGCCTGCGGGGAGATGTGATAACGCTTAAACAGACGaa < 1:632772/52‑1 (MQ=255) ctcAAGAATGACCGTCTGCGGGGAGATGTGATAACGCTTAAACAGACGaataat > 1:85443/1‑54 (MQ=255) ctcAAGAATGACCGTCTGCGGGGAGATGTGATAACGCTTAAACAGACGaataat > 1:1287460/1‑54 (MQ=255) gACCGCCTGCGGGGAGATGTGATAACGCTTAAACAGACGAATAATACGCCCGGCAATATTCTTTTgcag > 1:2536684/1‑69 (MQ=255) aCCGCCTGCGGGGAGATGTGATAACGCTTAAACAGACGAATAATCCGCCCGGCAATATTCTTTTGcagcag < 1:3309271/71‑1 (MQ=255) ccGCCTGCGGGGAGATGTGATAACGCTTAAACAGACGAATAATCCGCCCGGCAATATTCTTTTGcagcag < 1:3314134/70‑1 (MQ=255) ccTGCGGGGAGATGTGATAACGCTTAAACAGACGAATAATCCGCCCGGCAATATTCTTTTGCAGCAGCGTg < 1:1249529/71‑1 (MQ=255) | GCTCGATGTTGTACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATGACCGCCTGCGGGGAGATGTGATAACGCTTAAACAGACGAATAATCCGCCCGGCAATATTCTTTTGCAGCAGCGTG > W3110S.gb/2521394‑2521533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |