| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,526,348 | T→C | 9.7% | intergenic (‑230/‑29) | gltX ← / → valU | glutamyl‑tRNA synthetase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,526,348 | 0 | T | C | 9.7% | 104.0 / 4.4 | 41 | intergenic (‑230/‑29) | gltX/valU | glutamyl‑tRNA synthetase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (16/21); new base C (2/2); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGA > W3110S.gb/2526288‑2526417 | ttGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATaa > 1:516820/1‑71 (MQ=255) gCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACCCTCCCTATaa < 1:564819/69‑1 (MQ=255) gCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACCCTCCCTATaa < 1:2607287/69‑1 (MQ=255) ttAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGc > 1:1311192/1‑70 (MQ=255) tttACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCta < 1:682834/59‑1 (MQ=255) cgaacgaaCAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGa > 1:3548916/1‑60 (MQ=255) cgaacgaaCAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGa > 1:1194269/1‑60 (MQ=255) actaacAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCggg < 1:1242658/68‑1 (MQ=255) ctaacAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCtat < 1:747890/47‑1 (MQ=255) aacAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACCCTCCCTATAATGCGACTCCACACAGCg > 1:2837754/1‑66 (MQ=255) aacAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACCCTCCCTATAATGCGACTCCACACAGCg > 1:2720154/1‑66 (MQ=255) acAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGt < 1:1755375/70‑1 (MQ=255) aTTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGAtt < 1:3344937/71‑1 (MQ=255) aTTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGAtt < 1:3065540/71‑1 (MQ=255) aTTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGAtt < 1:1834156/71‑1 (MQ=255) aTTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGAtt < 1:1482690/71‑1 (MQ=255) ttCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGTGTGATTAg > 1:1121020/1‑71 (MQ=255) cTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAg > 1:3038115/1‑69 (MQ=255) cTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAg > 1:512751/1‑69 (MQ=255) tAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCa < 1:2009428/70‑1 (MQ=255) tAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCa < 1:4075201/70‑1 (MQ=255) tAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCa < 1:2957446/70‑1 (MQ=255) gAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCt < 1:1759724/71‑1 (MQ=255) gAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCt > 1:1778419/1‑71 (MQ=255) aaaTGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTgg < 1:1968228/71‑1 (MQ=255) aaTGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGc < 1:902082/67‑1 (MQ=255) aaTGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGc < 1:3133157/67‑1 (MQ=255) aaTGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGc < 1:3210523/67‑1 (MQ=255) aaTGCGTT‑ACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTgg < 1:1514004/69‑1 (MQ=255) gTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGc < 1:399717/71‑1 (MQ=255) ttGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTg < 1:301794/50‑1 (MQ=255) ttGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCa > 1:1156894/1‑71 (MQ=255) tGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTg < 1:315223/49‑1 (MQ=255) tGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTg < 1:2745252/49‑1 (MQ=255) gACTCATTTTGAACTCTCCCTATAATGGGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCAcc > 1:3480549/1‑71 (MQ=255) gACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGTGAGAGCAcc > 1:2993748/1‑71 (MQ=255) gACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCAcc > 1:3191373/1‑71 (MQ=255) ttGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGga > 1:3011500/1‑56 (MQ=255) ttGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCa > 1:1344565/1‑61 (MQ=255) ttGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCAc > 1:3112586/1‑62 (MQ=255) ttGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCAc > 1:1279647/1‑62 (MQ=255) ctctCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAagga < 1:1912479/71‑1 (MQ=255) | TTGCTTAAATTTACGACGAACGAACAATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGA > W3110S.gb/2526288‑2526417 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |