| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,550,560 | A→G | 33.3% | C166R (TGC→CGC) | yfeT ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,550,560 | 0 | A | G | 33.3% | 30.9 / 24.2 | 30 | C166R (TGC→CGC) | yfeT | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (8/12); new base G (3/7); total (11/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGATTTGTACATCGCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAGATCGCGCCCCACCAGCGCC > W3110S.gb/2550492‑2550618 | aGCGATTTGTACATCGCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCggg > 1:186195/1‑71 (MQ=255) gCGATTTGTACATCGCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCATGc < 1:3608567/71‑1 (MQ=255) gCGATTTGTACATCGCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGc < 1:528913/71‑1 (MQ=255) tGTACATCGCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCAcgc < 1:16245/70‑1 (MQ=255) cATCGCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGa > 1:391191/1‑68 (MQ=255) gCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGATAGCCaa > 1:2147390/1‑71 (MQ=255) ccTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCAcgcg < 1:1108312/62‑1 (MQ=255) ttttttCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGAt > 1:3461158/1‑62 (MQ=255) tttttCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAAttt > 1:1536714/1‑70 (MQ=255) ttttCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAAtttt < 1:1150196/70‑1 (MQ=255) ttttCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAAtttt < 1:429755/70‑1 (MQ=255) ttttCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACCCGATAGCCAAtttt < 1:3804924/70‑1 (MQ=255) ccTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGATAGCCAATTTTCATCAGTTTg < 1:110474/71‑1 (MQ=255) aaaCGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGATAGCCAATTTTCATCAGTTTGAAc < 1:959059/68‑1 (MQ=255) aaaCGGTGGCTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGATAGCCAATTTTCATCAGTTTGAAc < 1:120677/68‑1 (MQ=255) aaaCGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACg > 1:3770379/1‑69 (MQ=255) aaaCGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGa > 1:4035713/1‑70 (MQ=255) aCGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAg < 1:2229701/71‑1 (MQ=255) cTTGTACGTGCGTGTCGGCTTCGCGTGCCACGCGATAGCCAATTTTCATCAGTTTGAACGAc < 1:2978882/62‑1 (MQ=255) cTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGAc < 1:2790246/62‑1 (MQ=255) cTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGAc < 1:953931/62‑1 (MQ=255) cTTGTACGTGCGTGTCGGCTTCGCGGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGAc < 1:3547696/62‑1 (MQ=255) cgtgcgtgTCGGCTTCGCAGGCCACGCGATAGCCAATTTtca > 1:2051613/1‑42 (MQ=255) cgtgcgtgTCGGCTTCGCAGGCCACGCGATAGCCAATTTtc > 1:471480/1‑41 (MQ=255) tgcgtgTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAg < 1:2223402/56‑1 (MQ=255) tgcgtgTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAg < 1:922390/56‑1 (MQ=255) cgtgTCGGCTTCGCGGGCCACGCGATAGCCAATTTtcatc > 1:2588959/1‑40 (MQ=255) cgtgTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAGATCGCGCCCCACCAgcg > 1:852508/1‑71 (MQ=255) tgtCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAGATCGCGCCCCACCAgcgca < 1:4029180/71‑2 (MQ=255) tgtCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAGATCGCGCCCCACCAGCGcc < 1:550936/71‑1 (MQ=255) ttCGCGGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAGa > 1:1386696/1‑46 (MQ=255) | AGCGATTTGTACATCGCCTTTTTTCAACGCCTGAGAAACGGTGGCTTGTACGTGCGTGTCGGCTTCGCAGGCCACGCGATAGCCAATTTTCATCAGTTTGAACGACAGATCGCGCCCCACCAGCGCC > W3110S.gb/2550492‑2550618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |