| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,611,108 | T→C | 20.0% | F185L (TTC→CTC) | hyfR → | DNA‑binding transcriptional activator, formate sensing |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,611,108 | 0 | T | C | 20.0% | 115.2 / 25.6 | 55 | F185L (TTC→CTC) | hyfR | DNA‑binding transcriptional activator, formate sensing |
| Reads supporting (aligned to +/- strand): ref base T (35/9); new base C (7/4); total (42/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGG > W3110S.gb/2611040‑2611173 | aTGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCAtttttt < 1:987657/70‑1 (MQ=255) cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGt > 1:1337538/1‑71 (MQ=255) cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGt > 1:2091509/1‑71 (MQ=255) aGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:2531537/1‑71 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag > 1:3881936/1‑71 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag > 1:3175206/1‑71 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACCTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag > 1:2913659/1‑71 (MQ=255) tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGc > 1:3847707/1‑63 (MQ=255) tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGc > 1:2714646/1‑63 (MQ=255) tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGc > 1:1349860/1‑63 (MQ=255) tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGc > 1:1647225/1‑63 (MQ=255) ttGATCTCTACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcagtcag > 1:2550814/1‑70 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcagtcag > 1:2995935/1‑70 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcagtaag > 1:2852814/1‑70 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTtctc > 1:271255/1‑51 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGTCTTcagtcagt < 1:3969112/69‑1 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTcagtcagt < 1:2961327/69‑1 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATACATCATTTTTTCGGTCTGGCTTcagtcagt < 1:622336/69‑1 (MQ=255) aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcagtcag < 1:3508646/67‑1 (MQ=255) ctcGACGATCTGATCGCTGACGTCGCTCGTTAGATCCATCATTTTTTCGGTc > 1:3040262/1‑52 (MQ=255) ctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCTGTc > 1:2319757/1‑52 (MQ=255) ctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:3617180/1‑52 (MQ=255) ctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:1470036/1‑52 (MQ=255) tcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtc > 1:2781383/1‑56 (MQ=255) tcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtc < 1:692676/62‑1 (MQ=255) tcGACGATCTGATCGCTGACGTAGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTa > 1:36699/1‑71 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATgg < 1:1358944/67‑1 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTa > 1:903565/1‑69 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACt > 1:3581261/1‑71 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACt > 1:3397646/1‑71 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACt > 1:157938/1‑71 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGACTTCAGTCAGTATGGTACt > 1:2003041/1‑71 (MQ=255) acgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATgg < 1:4084192/66‑1 (MQ=255) aTCTGATCGCTGACGTCGCTCGTGAGATCCATCAttttt < 1:730751/39‑1 (MQ=255) aTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:1226536/51‑1 (MQ=255) aTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:3909758/51‑1 (MQ=255) aTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:715147/58‑1 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTc > 1:461648/1‑39 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTc > 1:263302/1‑39 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTc > 1:3652393/1‑39 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGa > 1:938102/1‑71 (MQ=255) aTCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcagt < 1:1109764/50‑1 (MQ=255) tCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAAc < 1:110549/71‑1 (MQ=255) tCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAAc < 1:2455869/71‑1 (MQ=255) ccATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc > 1:1249191/1‑38 (MQ=255) ccATCATTTTTTCGGTCTGGCTTAAGTCAGTATGGTAc > 1:2395452/1‑38 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGaa > 1:3984046/1‑60 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGaa > 1:1007387/1‑60 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGaa > 1:1574333/1‑60 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgt > 1:1332109/1‑71 (MQ=255) attattTCGGTCTGGCTTCAGTCAGTATGGTACTCGGCGATCATCGAAAGaa > 1:2264385/1‑52 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgtg > 1:382558/1‑70 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:2531065/1‑71 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:2539190/1‑71 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:594659/1‑71 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:2034941/1‑71 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:1152182/1‑71 (MQ=255) atTTTTTCGGTCTGGCTTCAGTAAGTATGGTACTGGGCGa > 1:1014456/1‑40 (MQ=255) atTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:3200117/1‑71 (MQ=255) tttttCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGt < 1:3396825/58‑1 (MQ=255) tttttCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGt < 1:2858094/58‑1 (MQ=255) | ATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGG > W3110S.gb/2611040‑2611173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |