| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,655,114 | C→T | 28.0% | intergenic (‑100/+78) | pepB ← / ← yfhJ | aminopeptidase B/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,655,114 | 0 | C | T | 28.0% | 36.7 / 14.6 | 25 | intergenic (‑100/+78) | pepB/yfhJ | aminopeptidase B/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (9/9); new base T (2/5); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCAT > W3110S.gb/2655045‑2655181 | cAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGcccc < 1:451941/71‑1 (MQ=255) ttCCGCTAAATGATTCGCGTTGCAGGAATGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAtgt < 1:995701/70‑1 (MQ=255) ttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTAtgt < 1:2903872/70‑1 (MQ=255) ttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTAtgt < 1:3443801/70‑1 (MQ=255) ttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTAtgt < 1:1723413/70‑1 (MQ=255) ttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAtgt < 1:977670/70‑1 (MQ=255) gCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTg < 1:901668/71‑1 (MQ=255) cTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTgg < 1:1824996/71‑1 (MQ=255) aaTGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGcccc < 1:3990747/42‑1 (MQ=255) aaTGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGcccc < 1:2623219/42‑1 (MQ=255) tGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTATGTGa > 1:73127/1‑62 (MQ=255) tGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTATGTGa > 1:3063101/1‑62 (MQ=255) tGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAg > 1:2887625/1‑42 (MQ=255) tGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGACCCAg > 1:2489845/1‑42 (MQ=255) aTTCTCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATa < 1:1085636/47‑1 (MQ=255) aGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAgcgc < 1:1220512/66‑1 (MQ=255) aaGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGaa > 1:2159526/1‑56 (MQ=255) aGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAT‑ACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCa > 1:2384228/1‑71 (MQ=255) aGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAT‑ACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCa > 1:2379522/1‑71 (MQ=255) tgagtgaAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcggcg > 1:2135826/1‑71 (MQ=255) tgaAGCCTCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAgcg < 1:319456/47‑1 (MQ=255) tgaAGCCTCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAgcg < 1:1924045/47‑1 (MQ=255) tgaAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCa < 1:3874684/58‑1 (MQ=255) tgaAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCa < 1:3880176/58‑1 (MQ=255) tgaAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCa < 1:2024372/58‑1 (MQ=255) gaAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAg > 1:2234661/1‑71 (MQ=255) aaGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAg > 1:389190/1‑70 (MQ=255) gCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCAt > 1:2981539/1‑71 (MQ=255) | CAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCAT > W3110S.gb/2655045‑2655181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |