| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,655,568 | C→T | 12.9% | G58S (GGT→AGT) | fdx ← | [2Fe‑2S] ferredoxin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,655,568 | 0 | C | T | 12.9% | 170.2 / 14.7 | 62 | G58S (GGT→AGT) | fdx | [2Fe‑2S] ferredoxin |
| Reads supporting (aligned to +/- strand): ref base C (33/21); new base T (3/5); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
AGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGA > W3110S.gb/2655504‑2655633 | aGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacg < 1:699014/71‑1 (MQ=255)aGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacg < 1:190360/71‑1 (MQ=255) cccAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaac > 1:528606/1‑70 (MQ=255) cccAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaac > 1:650107/1‑70 (MQ=255) ccAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaacga < 1:3698752/71‑1 (MQ=255) ggCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATg > 1:2236295/1‑70 (MQ=255) ggCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCATCACGAACGATg > 1:1703125/1‑70 (MQ=255) tGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCACGAACGATGCAGt < 1:258515/69‑1 (MQ=255) tGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCACGAACGATGCAGt < 1:768248/69‑1 (MQ=255) tGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCACGAACGATGCAGt < 1:1352100/69‑1 (MQ=255) tCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGc < 1:1784140/70‑1 (MQ=255) tGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaacg < 1:2660210/53‑1 (MQ=255) tGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaacg < 1:2546190/53‑1 (MQ=255) tGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATg < 1:889494/56‑1 (MQ=255) tGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATg < 1:429781/56‑1 (MQ=255) tGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAggt < 1:2612273/67‑1 (MQ=255) tcgtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAggtgg > 1:3134382/1‑67 (MQ=255) tcgtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAggtgg > 1:2307952/1‑67 (MQ=255) tcgtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGgtggtg > 1:2026937/1‑69 (MQ=255) tcgtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGc > 1:3923236/1‑70 (MQ=255) tcgtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCa < 1:300637/71‑1 (MQ=255) tcgtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCa < 1:1416477/71‑1 (MQ=255) tcgtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCa < 1:1947872/71‑1 (MQ=255) tcgtcTACCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAggtgg > 1:214671/1‑67 (MQ=255) gtcTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaac < 1:2807685/48‑1 (MQ=255) ttCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCacgaacga > 1:2192265/1‑47 (MQ=255) ttCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCacgaacga > 1:2925935/1‑47 (MQ=255) ttCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCacgaacga > 1:1361293/1‑47 (MQ=255) ttCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCACGAACGATGCAGTGGCa < 1:933078/57‑1 (MQ=255) ttCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACTTTCACGAACGATGCAGTGGCa < 1:2585239/57‑1 (MQ=255) ccTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCa > 1:3531580/1‑38 (MQ=255) ccTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCa > 1:3583203/1‑38 (MQ=255) ccTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCa > 1:618770/1‑38 (MQ=255) ccTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCa > 1:2382305/1‑38 (MQ=255) ccTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGca < 1:701306/68‑1 (MQ=255) ccTGCTCTGAGCTTTACGGCAGTGAGTCAAAACCTTCa > 1:3177334/1‑38 (MQ=255) tGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGGACGATGCAGTGGCAGGTGGTGCAAGCACAGGa > 1:2201937/1‑71 (MQ=255) tGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGa > 1:1029180/1‑71 (MQ=255) gatctGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGAt > 1:1059308/3‑43 (MQ=255) gCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGAt > 1:2768302/1‑43 (MQ=255) tGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAATCACAGGAttt < 1:564962/69‑1 (MQ=255) tGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGAttttt > 1:885245/1‑71 (MQ=255) tGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGAttttt > 1:2518969/1‑71 (MQ=255) aGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGt > 1:2027664/1‑42 (MQ=255) gAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCaa > 1:2279869/1‑70 (MQ=255) aGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTcaca > 1:808806/1‑58 (MQ=255) aGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCa < 1:3487647/68‑1 (MQ=255) tCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGc < 1:435159/59‑1 (MQ=255) tCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGc < 1:26241/59‑1 (MQ=255) tCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGc < 1:2804244/59‑1 (MQ=255) tCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAAtctc > 1:213017/1‑71 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGAt > 1:482944/1‑46 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGAt > 1:386980/1‑46 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGAt > 1:3518578/1‑46 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGAt > 1:1506209/1‑46 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCgt > 1:2426381/1‑59 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCgt > 1:949206/1‑59 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCgt > 1:991543/1‑59 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCg > 1:2008457/1‑58 (MQ=255) aaaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGa > 1:3819673/1‑71 (MQ=255) aaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGcac < 1:3963476/40‑1 (MQ=255) aaaCCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGcac < 1:1299552/40‑1 (MQ=255) cTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGAttttt > 1:2179205/1‑45 (MQ=255) | AGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGA > W3110S.gb/2655504‑2655633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |