| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,729,684 | T→C | 23.7% | noncoding (130/1542 nt) | rrsG ← | 16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,729,684 | 0 | T | C | 23.7% | 64.5 / 20.4 | 38 | noncoding (130/1542 nt) | rrsG | 16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (18/11); new base C (6/3); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
CTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGT > W3110S.gb/2729616‑2729748 | ctTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTc > 1:3277238/1‑71 (MQ=39) cTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACa > 1:3926454/1‑71 (MQ=39) ttGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCCAGACAt > 1:78161/1‑71 (MQ=255) gCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACAt < 1:1292813/69‑1 (MQ=40) gCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCGAGACAt < 1:521596/69‑1 (MQ=255) cATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCCAGACATTACTCa > 1:318397/1‑71 (MQ=255) aTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGa < 1:3750685/61‑1 (MQ=37) aTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGa < 1:3603485/61‑1 (MQ=37) ttATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACAt < 1:1612396/63‑1 (MQ=35) ttATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACAt < 1:2275225/63‑1 (MQ=35) aTGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCAccc > 1:1958343/1‑70 (MQ=35) aTGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCAcc > 1:646228/1‑69 (MQ=35) aTGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCg > 1:2730326/1‑71 (MQ=34) cGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACAt < 1:2161991/58‑1 (MQ=35) ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACa > 1:2068210/1‑52 (MQ=35) ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACa > 1:3626/1‑52 (MQ=35) aGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTc > 1:1759327/1‑63 (MQ=35) aGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCCCTc > 1:3881322/1‑71 (MQ=21) aGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTc > 1:3876822/1‑71 (MQ=34) tttCCAGTAGTTATCCCCCTCCATCAGGCAGCTTcc < 1:3996584/36‑1 (MQ=40) tttCCAGTAGTTATCCCCCTCCATCAGGCAGCTTcc < 1:3809727/36‑1 (MQ=40) tagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAagcagc > 1:28900/1‑70 (MQ=40) agtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAagcagc > 1:3530478/1‑69 (MQ=40) gtTATCCCCCTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAg > 1:2712033/1‑71 (MQ=255) aTCCCCCTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctg > 1:2171327/1‑71 (MQ=255) aTCCCCCTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctg > 1:3967321/1‑71 (MQ=255) ccTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTcc < 1:1422409/71‑1 (MQ=255) ccTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCCTCAGCGAAGCAGCAAGCTGCTTcc < 1:895297/71‑1 (MQ=14) tCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctg < 1:1762241/60‑1 (MQ=255) cAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTc > 1:547212/1‑39 (MQ=40) cAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctgct > 1:2707776/1‑61 (MQ=255) cAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgc > 1:3615032/1‑57 (MQ=255) aGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTc > 1:3885263/1‑62 (MQ=255) aGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTc > 1:3976572/1‑62 (MQ=255) aGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTc > 1:1660244/1‑62 (MQ=255) aGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCg > 1:3528102/1‑71 (MQ=255) ggCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGt < 1:583869/71‑1 (MQ=255) cTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGt < 1:3157995/66‑1 (MQ=255) | CTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGT > W3110S.gb/2729616‑2729748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |