| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,791,255 | G→A | 45.1% | G443S (GGC→AGC) | gabD → | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,791,255 | 0 | G | A | 45.1% | 8.4 / 34.7 | 31 | G443S (GGC→AGC) | gabD | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (12/5); new base A (10/4); total (22/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGA > W3110S.gb/2791189‑2791316 | cGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCat < 1:156431/71‑1 (MQ=255) tGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCatta > 1:1792980/1‑71 (MQ=255) gTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAATGAAg > 1:491780/1‑70 (MQ=255) gTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAATGAAg > 1:3970782/1‑70 (MQ=255) gTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAATGAAg > 1:1910320/1‑70 (MQ=255) gTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAATGAAg > 1:214142/1‑70 (MQ=255) cTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAATg > 1:1804328/1‑65 (MQ=255) cTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAATGaa > 1:947669/1‑67 (MQ=255) cTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAATGaa > 1:2322060/1‑67 (MQ=255) ttCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAAt > 1:1496445/1‑63 (MQ=255) ttCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGaa > 1:3428722/1‑66 (MQ=255) ccGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGc < 1:706537/69‑1 (MQ=255) gTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGtt > 1:855190/1‑71 (MQ=255) ggCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAAt > 1:140962/1‑54 (MQ=255) ggCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAAt > 1:1448623/1‑54 (MQ=255) ggCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCAGCATTATTTCCAAt > 1:1560682/1‑54 (MQ=255) cGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAg > 1:2173038/1‑56 (MQ=255) cGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAg > 1:107449/1‑56 (MQ=255) cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAg > 1:3888236/1‑41 (MQ=255) cGGCATCGTCGGCATCAATACCAGCATTATTTCCAATCAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCg < 1:4026653/70‑1 (MQ=255) ggCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCggg < 1:2002874/71‑1 (MQ=255) gtcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTgg > 1:767039/1‑70 (MQ=255) gtcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGGTCGGCGGCATCAAAGCCTCGGGTCTggg > 1:56846/1‑71 (MQ=255) ggCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCg > 1:3980308/1‑71 (MQ=255) tCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTg > 1:906657/1‑69 (MQ=255) tCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGa < 1:1660186/70‑1 (MQ=255) tCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCAGCATCAAAGCCTCGGGTCTGGGTCGTg > 1:3957144/1‑69 (MQ=255) aTACCAGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGa < 1:848804/67‑1 (MQ=255) tACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGcc < 1:3184921/49‑1 (MQ=255) tACCAGCATTATTTCCAATGAAGTGGCCCCGTTcggc < 1:369263/37‑1 (MQ=255) tACCAGCATTATTTCCAATGAAGTGGCCCCGTTcggc < 1:4004859/37‑1 (MQ=255) | CGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGA > W3110S.gb/2791189‑2791316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |