| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,860,149 | G→T | 33.3% | A22S (GCA→TCA) | ygbJ → | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,860,149 | 0 | G | T | 33.3% | 83.1 / 29.4 | 45 | A22S (GCA→TCA) | ygbJ | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (14/16); new base T (8/7); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGC > W3110S.gb/2860087‑2860207 | tGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCa < 1:2870942/71‑1 (MQ=255)tGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:941181/71‑1 (MQ=255)tGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:1335042/71‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:3146235/1‑64 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:2093760/1‑64 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:1587331/1‑64 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:1999870/1‑70 (MQ=255) gAAAACGGGATATGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:1385880/1‑64 (MQ=255) aaaaCGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCata > 1:3554122/1‑71 (MQ=255) aaaaCGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCata > 1:2305871/1‑71 (MQ=255) aaaaCGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCat < 1:2356533/70‑1 (MQ=255) cGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCATATGt > 1:1787432/1‑70 (MQ=255) cGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCATATGTc > 1:3593994/1‑71 (MQ=255) aTCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcatca > 1:997437/1‑55 (MQ=255) gAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCATATg < 1:2531469/61‑1 (MQ=255) tttCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCt < 1:956028/71‑1 (MQ=255) tttCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCt < 1:1531602/71‑1 (MQ=255) tttCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCt < 1:748801/71‑1 (MQ=255) tCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:1056570/1‑46 (MQ=255) tCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:1608859/1‑46 (MQ=255) tCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCAc > 1:1188421/1‑47 (MQ=255) tCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCAc > 1:3055827/1‑47 (MQ=255) tCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCAc > 1:2571609/1‑47 (MQ=255) cATGTCGGTATCGTTGCCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCttt < 1:3209543/70‑1 (MQ=255) aTGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTc < 1:3834676/70‑1 (MQ=255) ggTATCGTTGGCTTAGGGTCAATGGGAATGGGTGCATCACTGTCat < 1:1465252/46‑1 (MQ=255) ggTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCat < 1:878256/46‑1 (MQ=255) gTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCat < 1:185889/45‑1 (MQ=255) tATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACt < 1:3065511/39‑1 (MQ=255) tATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACt < 1:2280368/39‑1 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCATATGTCCGCGCAg > 1:2286914/1‑52 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCATCACTGTCATATGTCCGCGCAGGTCTTTCTACCTggg > 1:3116342/1‑67 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCata > 1:1088765/1‑41 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCt < 1:674641/56‑1 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTAc > 1:192534/1‑62 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTAc > 1:3987345/1‑62 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGgcg < 1:992234/70‑1 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGgcg > 1:680623/1‑70 (MQ=255) gTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGgcg > 1:2434662/1‑70 (MQ=255) ttGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCT‑GGGCGCAg < 1:2161246/71‑1 (MQ=255) ggCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAg < 1:1209010/70‑1 (MQ=255) ggCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAg < 1:3973728/70‑1 (MQ=255) ggCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGa > 1:3918666/1‑71 (MQ=255) aGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTg < 1:432919/70‑1 (MQ=255) gggTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGt > 1:1757822/1‑45 (MQ=255) gggTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGt > 1:3692568/1‑45 (MQ=255) aatgggaatgggAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGc < 1:1045389/70‑1 (MQ=255) atgggaatgggaGCATCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCaa < 1:130203/71‑1 (MQ=255) gggaatgggaGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATg > 1:1430763/1‑71 (MQ=255) ggaatgggaGCATCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGc > 1:1255845/1‑71 (MQ=255) ggaatgggaGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGc > 1:354604/1‑71 (MQ=255) atgggaGCATCACTGTCATATGTCCGCGCAGGTCTTTCTAc > 1:2649214/1‑41 (MQ=255) | TGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGC > W3110S.gb/2860087‑2860207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |