| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,863,108 | T→C | 28.6% | L73L (TTA→CTA) | ygbM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,863,108 | 0 | T | C | 28.6% | 57.3 / 30.4 | 42 | L73L (TTA→CTA) | ygbM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (17/13); new base C (5/7); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
GAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAT > W3110S.gb/2863039‑2863174 | gAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt > 1:2470169/1‑70 (MQ=255) tCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTa < 1:2645587/61‑1 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTccc > 1:2569732/1‑71 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTccc > 1:3401900/1‑71 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTcc > 1:1007222/1‑70 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCg < 1:2265826/70‑1 (MQ=255) cTGGCGCTGTTCAATACAGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACg > 1:1040378/1‑71 (MQ=255) gcTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAg < 1:1707176/70‑1 (MQ=255) ttCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt < 1:1819371/43‑1 (MQ=255) tCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCAGGa > 1:1792732/1‑59 (MQ=255) cAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAg > 1:815548/1‑71 (MQ=255) cAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAg > 1:1965022/1‑71 (MQ=255) cAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAg > 1:1275717/1‑71 (MQ=255) cAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAg > 1:3618664/1‑71 (MQ=255) aTACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:3219058/71‑1 (MQ=255) aTACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:1870110/71‑1 (MQ=255) aTACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:160756/71‑1 (MQ=255) tACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTg < 1:1343230/59‑1 (MQ=255) aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:3831327/1‑71 (MQ=255) ccGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGAACGTGAGCACGAAGCacac > 1:488144/1‑71 (MQ=255) ccGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATC‑‑CCCTTCCCGGACGTGAGCACGAAGCACACg > 1:1902305/1‑70 (MQ=255) cTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:214718/71‑1 (MQ=255) cTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:3917810/71‑1 (MQ=255) gagaTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATc < 1:2196643/71‑1 (MQ=255) agaTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGTCGTGAGCACg < 1:1651989/53‑1 (MQ=255) agaTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:705964/71‑1 (MQ=255) agaTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:1848586/71‑1 (MQ=255) agaTATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:1583549/71‑1 (MQ=255) agaTATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:3026955/71‑1 (MQ=255) agaTATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:3031664/71‑1 (MQ=255) atTAATGCGGGGGAATGGGGATTATCCGCCCTTccc > 1:2800144/1‑36 (MQ=255) atTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCa > 1:3975763/1‑47 (MQ=255) atTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:1744661/1‑56 (MQ=255) atTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:76319/1‑56 (MQ=255) atTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCacac > 1:2679386/1‑57 (MQ=255) atTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTCAGCACGAAGCacac > 1:3955027/1‑57 (MQ=255) atTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGa > 1:2014220/1‑62 (MQ=255) aTGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGc < 1:1813050/70‑1 (MQ=255) gAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACg < 1:3310190/37‑1 (MQ=255) tGGGGATTATCCGCCCTTCCCGGACGTGAGCACGa < 1:2828241/35‑1 (MQ=255) tGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGc < 1:1414201/59‑1 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAt > 1:1023223/1‑71 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAt > 1:3300476/1‑71 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAt > 1:2851428/1‑71 (MQ=255) | GAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAT > W3110S.gb/2863039‑2863174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |