| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,865,292 | G→A | 11.8% | Q267* (CAG→TAG) | rpoS ← | RNA polymerase, sigma S (sigma 38) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,865,292 | 0 | G | A | 11.8% | 144.1 / 9.1 | 51 | Q267* (CAG→TAG) | rpoS | RNA polymerase, sigma S (sigma 38) factor |
| Reads supporting (aligned to +/- strand): ref base G (20/25); new base A (2/4); total (22/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCTTCGTAC > W3110S.gb/2865224‑2865362 | gAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGaa > 1:1604678/1‑71 (MQ=255) cAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCt > 1:3211674/1‑71 (MQ=255) cAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTT‑GCGCAAACGGCGCAGGCCTTCAACCTGAATCTg > 1:3798942/1‑71 (MQ=255) gcgcTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTgg > 1:348095/1‑71 (MQ=255) gcgcTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTgg > 1:1414014/1‑71 (MQ=255) cGATATTCAGCCCCTGCTTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac < 1:3244086/70‑1 (MQ=255) cGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac < 1:2857687/70‑1 (MQ=255) gATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAaca < 1:2826238/70‑1 (MQ=255) atTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGtt > 1:1409745/1‑71 (MQ=255) atTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGtt > 1:1794262/1‑71 (MQ=255) ttCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGtt < 1:1089986/70‑1 (MQ=255) ttCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTAAATCTGGCGAACACGtt < 1:879003/70‑1 (MQ=255) ttCAGCACCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTAAATCTGGCGAACACGtt < 1:2217518/70‑1 (MQ=255) aGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc < 1:3236030/70‑1 (MQ=255) aGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACg < 1:993231/71‑1 (MQ=255) tGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGtt < 1:3865701/61‑1 (MQ=255) gCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCCACCTGAATCTGGCGAACACGTTCACGGGTGAgg > 1:1171428/1‑71 (MQ=255) gCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAacac > 1:873318/1‑57 (MQ=255) gCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAacac > 1:1437550/1‑57 (MQ=255) gCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAgg > 1:163491/1‑71 (MQ=255) gCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAgg > 1:23529/1‑71 (MQ=255) cGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGc > 1:2123464/1‑71 (MQ=255) tttGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCa < 1:3869930/71‑1 (MQ=255) cAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAttt < 1:439155/71‑1 (MQ=255) aTTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACg < 1:2058220/71‑1 (MQ=255) cgcgCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc < 1:2499627/70‑1 (MQ=255) gcgcAAACGGCGCAGGCCTTCAACCTAAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTa < 1:2845170/71‑1 (MQ=255) aCGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc < 1:2059464/41‑1 (MQ=255) aCGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc < 1:1782436/41‑1 (MQ=255) aCGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc < 1:2611580/41‑1 (MQ=255) aCGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc < 1:2692801/41‑1 (MQ=255) aCGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc < 1:1466133/41‑1 (MQ=255) gcAGGCCTTCAACCTAAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAgt < 1:1828234/71‑1 (MQ=255) cTTCAACCTAAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGcc > 1:1871013/1‑71 (MQ=255) cTTCAACCTAAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGcc > 1:3611680/1‑71 (MQ=255) ttCAACCTGAATCTGGCGAACACGTTCACGTGTGAGGCCAATTTCACGACCTACATCTTcc < 1:2728185/61‑1 (MQ=255) ttCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTcc < 1:3015424/61‑1 (MQ=255) ttCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTcc < 1:1219743/61‑1 (MQ=255) ttCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTcc < 1:805007/61‑1 (MQ=255) ttCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTcc < 1:675351/61‑1 (MQ=255) ttCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTcc < 1:411966/61‑1 (MQ=255) cAAGCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCt > 1:2423343/1‑71 (MQ=255) cAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCt > 1:1509556/1‑71 (MQ=255) cAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCt > 1:1137296/1‑71 (MQ=255) cAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCt > 1:546129/1‑71 (MQ=255) cAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCt > 1:2134454/1‑71 (MQ=255) cAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCt > 1:224017/1‑71 (MQ=255) cTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAttt < 1:24339/38‑1 (MQ=255) cTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGtt < 1:1202644/61‑1 (MQ=255) cTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGtt < 1:154985/61‑1 (MQ=255) tGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTc > 1:1965851/1‑53 (MQ=255) gAATCTGGCGAACACGTTGACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCTTCGTAc > 1:1042275/1‑71 (MQ=255) | GAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACATCTTCCAGTGTTGCCGCTTCGTAC > W3110S.gb/2865224‑2865362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |