| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 192,898 | T→C | 23.8% | A9A (GCT→GCC) | frr → | ribosome recycling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 192,898 | 0 | T | C | 23.8% | 75.7 / 23.3 | 42 | A9A (GCT→GCC) | frr | ribosome recycling factor |
| Reads supporting (aligned to +/- strand): ref base T (13/19); new base C (3/7); total (16/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCT > W3110S.gb/192830‑192967 | aCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTg < 1:3784743/70‑1 (MQ=255)aCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTg < 1:266352/70‑1 (MQ=255)aCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTg < 1:3379873/70‑1 (MQ=255)aCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTg < 1:3779846/70‑1 (MQ=255) tGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAg > 1:1295815/1‑71 (MQ=255) gtctggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAg > 1:68951/1‑70 (MQ=255) tctggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTa > 1:3550963/1‑71 (MQ=255) ggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTa > 1:686066/1‑68 (MQ=255) gtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGa < 1:1562227/63‑1 (MQ=255) gtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGa < 1:1181333/63‑1 (MQ=255) gtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACg < 1:703356/69‑1 (MQ=255) tgactgaGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATg > 1:4065510/1‑70 (MQ=255) actgaGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGa > 1:298926/1‑70 (MQ=255) ctgaGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACa < 1:3581227/71‑1 (MQ=255) aCAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACa < 1:3146846/66‑1 (MQ=255) cAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCg < 1:57506/71‑1 (MQ=255) cAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACa < 1:3897588/65‑1 (MQ=255) cAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACa < 1:1059272/65‑1 (MQ=255) aGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTa < 1:1070066/71‑1 (MQ=255) ttttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATTCCGAAg < 1:2406094/49‑1 (MQ=255) ttttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGa < 1:2855959/71‑1 (MQ=255) tttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAg > 1:2193862/1‑69 (MQ=255) tttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAg > 1:3633287/1‑69 (MQ=255) ttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAg > 1:718020/1‑71 (MQ=255) cAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGc < 1:722480/70‑1 (MQ=255) aaGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACa < 1:947521/56‑1 (MQ=255) aTTCGTAACGTGATTCGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCaa < 1:3708320/71‑1 (MQ=255) aCGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCaa < 1:1772929/71‑1 (MQ=255) aCGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCaa < 1:1026406/71‑1 (MQ=255) aCGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCaa < 1:321521/71‑1 (MQ=255) aGCGATATCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCa > 1:476964/1‑70 (MQ=255) gCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaa < 1:1357070/70‑1 (MQ=255) gCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaa < 1:563560/70‑1 (MQ=255) cGATATCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaaaa < 1:1198227/71‑1 (MQ=255) atatCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCa < 1:1580787/58‑1 (MQ=255) tCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACg > 1:848383/1‑70 (MQ=255) aaGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAg > 1:1256362/1‑55 (MQ=255) aaGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACgg > 1:2077283/1‑69 (MQ=255) aaGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATAAGCaaaa > 1:1314338/1‑60 (MQ=255) aaGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCAAAATCAGCAAAATa > 1:1989613/1‑62 (MQ=255) aTGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACg < 1:2580408/61‑1 (MQ=255) cTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCt > 1:1551109/1‑71 (MQ=255) | ACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTGCT > W3110S.gb/192830‑192967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |