| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,886,205 | A→G | 40.6% | intergenic (‑330/+29) | ygcB ← / ← cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,886,205 | 0 | A | G | 40.6% | 13.7 / 35.9 | 32 | intergenic (‑330/+29) | ygcB/cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base A (12/7); new base G (6/7); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
CTTCCCGCGGCGTTCACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCA > W3110S.gb/2886136‑2886270 | cttcCCGCGGCGTTCACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGa > 1:3725242/1‑70 (MQ=255) cACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGa > 1:2426940/1‑56 (MQ=255) cACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGTGTAAtt > 1:806943/1‑71 (MQ=255) cGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTg < 1:1103369/71‑1 (MQ=255) cGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTg > 1:121167/1‑71 (MQ=255) cGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTAAATTTg < 1:662/71‑1 (MQ=255) tAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTGTCCg > 1:3842591/1‑71 (MQ=255) tCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAgg < 1:3894747/51‑1 (MQ=255) cAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAata > 1:804581/1‑71 (MQ=255) aTGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTa < 1:2687511/70‑1 (MQ=255) gctgctCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTAcc > 1:2677358/1‑70 (MQ=255) ctgctCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGttt < 1:1089104/47‑1 (MQ=255) ctgctCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCt > 1:2183547/1‑71 (MQ=255) ctgctCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCt > 1:505161/1‑71 (MQ=255) ctgctCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCt > 1:2882473/1‑71 (MQ=255) ctgctCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCt > 1:1714354/1‑71 (MQ=255) gctCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAggg > 1:3338060/1‑40 (MQ=255) ccAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAat < 1:464834/55‑1 (MQ=255) cAAAATAAACACGCCCCCATCATTTCTGGCAGAGGCGTTTAAt < 1:3941824/43‑1 (MQ=255) cAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCgtgt < 1:1648281/70‑1 (MQ=255) aaaaTAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTa < 1:3149195/70‑1 (MQ=255) aaaTAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTa < 1:2096858/69‑1 (MQ=255) tAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCca < 1:1286966/71‑1 (MQ=255) cccATCATTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATAttt < 1:1537212/44‑1 (MQ=255) catcatTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTAc > 1:999879/1‑44 (MQ=255) catcatTTCTGACAGAGGCGTTTAATTTTTCCGGCAata > 1:410782/1‑39 (MQ=255) atcatTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTAGTTTAACCCACATTCCCtttt > 1:2235511/1‑69 (MQ=255) tcatTTCTGACAGAGGCGTTTAATTTGTCCGGCAat < 1:4070232/36‑1 (MQ=255) tcatTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTcc < 1:3056810/63‑1 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAatat > 1:3898731/1‑36 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTa > 1:3619376/1‑39 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAg > 1:3885651/1‑70 (MQ=255) ttCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGcc > 1:3960500/1‑70 (MQ=255) tCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCa > 1:819058/1‑70 (MQ=255) | CTTCCCGCGGCGTTCACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCA > W3110S.gb/2886136‑2886270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |