| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,892,483 | A→G | 39.5% | K391E (AAA→GAA) | ygcN → | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,892,483 | 0 | A | G | 39.5% | 25.2 / 48.2 | 43 | K391E (AAA→GAA) | ygcN | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base A (13/13); new base G (10/7); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTG > W3110S.gb/2892424‑2892541 | aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAAcc > 1:1099214/1‑64 (MQ=255)aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAAcc > 1:1454994/1‑64 (MQ=255) tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTg < 1:2255190/62‑1 (MQ=255) cGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTg > 1:3463993/1‑71 (MQ=255) cGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTg > 1:3014201/1‑71 (MQ=255) cGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTg > 1:2919933/1‑71 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATaaa > 1:2136927/1‑47 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCa > 1:3676703/1‑56 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTa > 1:722040/1‑71 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTa < 1:320800/71‑1 (MQ=255) ttAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGcc < 1:1064448/64‑1 (MQ=255) aaTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAg > 1:2934881/1‑64 (MQ=255) aTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTa < 1:3378664/66‑1 (MQ=255) aTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTAt < 1:482667/67‑1 (MQ=255) gATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatca > 1:1820211/1‑71 (MQ=255) gATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatca > 1:3157155/1‑71 (MQ=255) gATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatca < 1:614610/71‑1 (MQ=255) gATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatca < 1:3259465/71‑1 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt < 1:1734892/71‑1 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt < 1:582865/71‑1 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt > 1:1502487/1‑71 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt < 1:3477947/71‑1 (MQ=255) atTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcat > 1:3070782/1‑66 (MQ=255) atTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt > 1:2362299/1‑70 (MQ=255) tttCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCt < 1:404413/60‑1 (MQ=255) tttCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCt < 1:1132385/60‑1 (MQ=255) tttCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTa > 1:119398/1‑71 (MQ=255) tttCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTa > 1:1468526/1‑71 (MQ=255) tttCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTa > 1:424578/1‑71 (MQ=255) tCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTgg > 1:1111601/1‑60 (MQ=255) tCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTgg > 1:1038212/1‑60 (MQ=255) tCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcg > 1:2755717/1‑71 (MQ=255) tCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcg > 1:3303128/1‑71 (MQ=255) aTTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcgtc < 1:3098054/66‑1 (MQ=255) aTTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcgtc < 1:1496873/66‑1 (MQ=255) aTTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcgtc < 1:2839306/66‑1 (MQ=255) tttATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCt < 1:1400525/50‑1 (MQ=255) tttATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCt < 1:350368/50‑1 (MQ=255) tttATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTGGTCACg < 1:9292/71‑1 (MQ=255) tttATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACg < 1:2673993/71‑1 (MQ=255) gATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtgtg < 1:2152187/71‑1 (MQ=255) cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatcat > 1:2038191/1‑48 (MQ=255) aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcgtc > 1:43227/1‑48 (MQ=255) aTAAACCTGTTCCACCGCTGCGCCAG‑TATTCTGGCATCATTTAcgtc > 1:931259/1‑47 (MQ=39) aaaCCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTa > 1:3805683/1‑42 (MQ=255) aaaCCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTa > 1:1123523/1‑42 (MQ=255) aaaCCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTa > 1:155675/1‑42 (MQ=255) | ACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTG > W3110S.gb/2892424‑2892541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |