| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,902,330 | A→T | 27.3% | intergenic (+300/+1073) | ygcE → / ← ygcF | predicted kinase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,902,330 | 0 | A | T | 27.3% | 50.2 / 22.2 | 33 | intergenic (+300/+1073) | ygcE/ygcF | predicted kinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (15/9); new base T (5/4); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
CTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACC > W3110S.gb/2902260‑2902398 | cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata < 1:500854/71‑1 (MQ=255) cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCg > 1:1999487/1‑71 (MQ=255) ttttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGt > 1:2294536/1‑70 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaa < 1:3556831/71‑1 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaa < 1:2833905/71‑1 (MQ=255) tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaaaa > 1:602806/1‑71 (MQ=255) tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaaaa > 1:3776665/1‑71 (MQ=255) tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACt < 1:3370794/71‑1 (MQ=255) tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACt < 1:3300847/71‑1 (MQ=255) tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACt < 1:1841910/71‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTc < 1:485131/70‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCa > 1:2037749/1‑71 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCa > 1:3809830/1‑71 (MQ=255) gATATCTTTATTCATAAGAACTATTCATCAACAGCGtata > 1:2471789/1‑40 (MQ=255) atatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTc < 1:1823922/62‑1 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATag < 1:305439/37‑1 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATag < 1:63281/37‑1 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaa > 1:300929/1‑70 (MQ=255) tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaaa > 1:51530/1‑70 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTAt > 1:1889275/1‑43 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACa > 1:277315/1‑70 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACa > 1:1056421/1‑70 (MQ=255) ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACa > 1:3005723/1‑68 (MQ=255) aTAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACa < 1:2011771/71‑1 (MQ=255) aGAACTATTCATCAACAGCGTATTGAGGCGGTTATGt > 1:4458/1‑37 (MQ=255) ttCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACt > 1:1991630/1‑39 (MQ=255) ttCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGc > 1:2985660/1‑46 (MQ=255) ttCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGc > 1:1294170/1‑46 (MQ=255) catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt > 1:1577936/1‑71 (MQ=255) atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt < 1:3804886/70‑1 (MQ=255) atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt < 1:2202897/70‑1 (MQ=255) aaCAGCGTATTGAGGCGGTTATGTAAAACCACTCAt < 1:2709766/36‑1 (MQ=255) aaCAGCGTATTGAGGCGGTTATGTAAAACCACTCAt < 1:2620405/36‑1 (MQ=255) aaCAGCGTATTGAGGCGGTTATGTAAAACCACTCAt < 1:2353232/36‑1 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAATCCACTCATTAGCCTCaaaa > 1:3111256/1‑47 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaaa > 1:3989080/1‑47 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttat > 1:486312/1‑70 (MQ=255) tattGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACaa < 1:3953891/49‑1 (MQ=255) taGAGGCGTTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAAcc < 1:167948/70‑1 (MQ=255) | CTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACC > W3110S.gb/2902260‑2902398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |