| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,927,326 | G→A | 17.0% | V148M (GTG→ATG) | sdaC → | predicted serine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,927,326 | 0 | G | A | 17.0% | 127.4 / 18.1 | 53 | V148M (GTG→ATG) | sdaC | predicted serine transporter |
| Reads supporting (aligned to +/- strand): ref base G (23/21); new base A (5/4); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
GAAAGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTATTC > W3110S.gb/2927257‑2927394 | gAAAGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGCTCGt > 1:943103/1‑71 (MQ=255)gAAAGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGt > 1:2315048/1‑71 (MQ=255)gAAAGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGt > 1:2926944/1‑71 (MQ=255)gAAAGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGt > 1:2922241/1‑71 (MQ=255) aGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg > 1:3897512/1‑71 (MQ=255) cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTa > 1:4019158/1‑71 (MQ=255) cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatga > 1:2556367/1‑71 (MQ=255) tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatgatg < 1:1951219/71‑1 (MQ=255) tctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGAc > 1:486209/1‑71 (MQ=255) tcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGAcc > 1:3235387/1‑70 (MQ=255) ccAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCg < 1:2036132/71‑1 (MQ=255) ccAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCg < 1:1991474/71‑1 (MQ=255) ccAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCg < 1:3693715/71‑1 (MQ=255) ccAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCg < 1:2801924/71‑1 (MQ=255) ccAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCg < 1:3358171/71‑1 (MQ=255) cAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGt > 1:1760012/1‑71 (MQ=255) cAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGt > 1:3472021/1‑71 (MQ=255) aGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGtt > 1:1043089/1‑71 (MQ=255) aGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGtt < 1:1450488/71‑1 (MQ=255) aGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGtt < 1:735116/71‑1 (MQ=255) tATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg < 1:1935262/48‑1 (MQ=255) tATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg < 1:3499934/48‑1 (MQ=255) aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCg > 1:3163694/1‑70 (MQ=255) tGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTat < 1:2256472/49‑1 (MQ=255) gACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt > 1:2348652/1‑70 (MQ=255) gACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt > 1:527748/1‑70 (MQ=255) aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCAt > 1:3720772/1‑56 (MQ=255) aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCgg > 1:1317274/1‑68 (MQ=255) gCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAg < 1:291626/70‑1 (MQ=255) gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatga > 1:3704759/1‑71 (MQ=255) gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTa > 1:695661/1‑38 (MQ=255) gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTa > 1:806092/1‑38 (MQ=255) cgTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGtt < 1:1115903/48‑1 (MQ=255) gTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGtt < 1:1772338/71‑1 (MQ=255) gTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGtt < 1:63846/71‑1 (MQ=255) cGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa > 1:3171465/1‑69 (MQ=255) aTTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc < 1:3621607/71‑1 (MQ=255) aTTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc < 1:1809156/71‑1 (MQ=255) aTTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc < 1:460346/71‑1 (MQ=255) cTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa > 1:1133513/1‑70 (MQ=255) tCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg < 1:3785991/71‑1 (MQ=255) tCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATggg > 1:258092/1‑69 (MQ=255) tCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg < 1:1347876/71‑1 (MQ=255) tgatcctgatcGTGGGTATGATGACCATCGTTCGCTTCgg > 1:1609940/1‑40 (MQ=255) tcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt < 1:3094019/61‑1 (MQ=255) tcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGAt < 1:1130956/61‑1 (MQ=255) ctgatcGTGGGTATTATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg > 1:2241553/1‑70 (MQ=255) ctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg > 1:176416/1‑70 (MQ=255) ctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg < 1:2003293/70‑1 (MQ=255) ctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg > 1:959743/1‑70 (MQ=255) ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg < 1:186146/70‑1 (MQ=255) atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTGTGGTAt < 1:521935/70‑1 (MQ=255) tcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTATTc > 1:2942943/1‑71 (MQ=255) | GAAAGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTATTC > W3110S.gb/2927257‑2927394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |