| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,939,281 | C→T | 45.5% | E207K (GAA→AAA) | ygdE ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,939,281 | 0 | C | T | 45.5% | 10.5 / 57.0 | 44 | E207K (GAA→AAA) | ygdE | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base T (10/10); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01 | |||||||||||
AGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTGGAACGACTCGGCGC > W3110S.gb/2939214‑2939350 | aGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATc < 1:3425928/71‑1 (MQ=255) gTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATc < 1:787549/70‑1 (MQ=255) tCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATcc > 1:2987274/1‑71 (MQ=255) ccAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATcc > 1:2774758/1‑70 (MQ=255) ccAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg > 1:4014226/1‑71 (MQ=255) cAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg > 1:589467/1‑70 (MQ=255) ccgccAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAgg < 1:3795491/71‑1 (MQ=255) aGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa > 1:1726376/1‑70 (MQ=255) cAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGTAATAAacac < 1:3570360/71‑1 (MQ=255) cAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAacac < 1:1230934/71‑1 (MQ=255) cAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAacac < 1:2229924/71‑1 (MQ=255) cgcCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGa < 1:240299/71‑1 (MQ=255) ccTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGa < 1:2012952/69‑1 (MQ=255) ccTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGa < 1:2812356/69‑1 (MQ=255) ccTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGa < 1:3565955/69‑1 (MQ=255) aaaTCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAAcaca > 1:3458733/1‑63 (MQ=255) aaaTCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGaa > 1:185941/1‑67 (MQ=255) aaaTCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGaa > 1:398554/1‑67 (MQ=255) aaaTCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGaa > 1:3016540/1‑67 (MQ=255) aaTCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGaaa > 1:3490888/1‑67 (MQ=255) aaTCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGaaa > 1:1582729/1‑67 (MQ=255) aaTCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGaaa > 1:1578086/1‑67 (MQ=255) aaTCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGaaa > 1:1417069/1‑67 (MQ=255) aaTCCACCGCCCACATCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGaaa > 1:312301/1‑67 (MQ=255) ccGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTcc < 1:831964/71‑1 (MQ=255) cGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCa < 1:3452182/71‑1 (MQ=255) acaTCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATa > 1:1176573/1‑44 (MQ=255) acaTCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATa > 1:3967539/1‑44 (MQ=255) caTCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGAAAt < 1:1654624/55‑1 (MQ=255) caTCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGAAAt < 1:826749/55‑1 (MQ=255) caTCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGAAAt < 1:1333635/55‑1 (MQ=255) caTCCCGTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGAAAt < 1:1446326/55‑1 (MQ=255) caTCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGaa > 1:2179020/1‑41 (MQ=255) caTCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGaa > 1:1904907/1‑41 (MQ=255) caTCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGaa > 1:118830/1‑41 (MQ=255) tCCCGTTCGCCAGGTGTTCATCCCACTCATCCGCAGTAATAAACACATGAAATGCCTCTTCCAGTTTg < 1:2523123/68‑1 (MQ=255) tCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTcc < 1:529397/62‑1 (MQ=255) ccGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGaaa > 1:2544650/1‑50 (MQ=255) gTTCGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTg > 1:3433096/1‑70 (MQ=255) gTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTgg < 1:3989544/71‑1 (MQ=255) gTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTgg < 1:66622/71‑1 (MQ=255) ttCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTGGa < 1:3115713/71‑1 (MQ=255) cGCCAGGCGTTTATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTGGAAc > 1:2491781/1‑71 (MQ=255) aGGCGTTTATCCCACTCATCCGCAGGAATAAacac < 1:3348529/35‑1 (MQ=255) cATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTGGAACGACTCGgcgc < 1:714788/70‑1 (MQ=255) | AGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGTTTGAGCGTGGAACGACTCGGCGC > W3110S.gb/2939214‑2939350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |