| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,953,831 | T→C | 22.5% | T277A (ACA→GCA) | recB ← | exonuclease V (RecBCD complex), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,953,831 | 0 | T | C | 22.5% | 73.7 / 20.4 | 40 | T277A (ACA→GCA) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (18/13); new base C (5/4); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
CCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGC > W3110S.gb/2953762‑2953896 | ccTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtc < 1:3321100/71‑1 (MQ=255) cGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtctc < 1:2477573/69‑1 (MQ=255) cGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtct > 1:1546300/1‑68 (MQ=255) cGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCtctt > 1:993783/1‑71 (MQ=255) cGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCtctt > 1:234446/1‑71 (MQ=255) cGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGCctct > 1:957496/1‑70 (MQ=255) cGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCAGCAACTGATAACTGTTTGTCtctt > 1:1038503/1‑71 (MQ=255) gTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCtcttc < 1:3096761/71‑1 (MQ=255) gtAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTCTGTCTCTTCTTCTg < 1:3802461/65‑1 (MQ=255) ctAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGt > 1:3450773/1‑55 (MQ=255) ctAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGt > 1:2474806/1‑55 (MQ=255) ctAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCtcttcttct < 1:1905535/65‑1 (MQ=255) ctAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAg > 1:422810/1‑71 (MQ=255) ctAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAg > 1:1994984/1‑71 (MQ=255) ctAAGAAACCCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAg > 1:378249/1‑71 (MQ=255) aaaCGCTGGGAGAATTTTTCCAGCGACTGCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCt < 1:1901565/71‑1 (MQ=255) aaaCGCTGGGAGAATTTTTCCAGCGACCCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCt < 1:3700670/71‑1 (MQ=255) aaCGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCt > 1:3675937/1‑70 (MQ=255) aaCGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCt > 1:4052942/1‑70 (MQ=255) aaCGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCt > 1:279004/1‑70 (MQ=255) cGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCtcttcttc > 1:610191/1‑56 (MQ=255) cGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCtcttcttc > 1:1397215/1‑56 (MQ=255) gCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATc > 1:3933464/1‑71 (MQ=255) tttCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCAt > 1:3574339/1‑71 (MQ=255) tttCCAGCGACTCCGGCAACTGATAACTGTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCa < 1:567505/70‑1 (MQ=255) tttCCAGCGACTCCGGCAACTGATAACTGTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCa < 1:251991/70‑1 (MQ=255) tttCCAGCGACTCCGGCAACTGATAACTGTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCa < 1:2475048/70‑1 (MQ=255) cGACTCCGGCAACTGATAACTGTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCt < 1:3214677/71‑1 (MQ=255) cTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATcc < 1:4061670/50‑1 (MQ=255) cTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATcc < 1:2424966/50‑1 (MQ=255) tGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGt > 1:1426152/1‑70 (MQ=255) tAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTaaa < 1:1964055/71‑1 (MQ=255) tAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTaaa < 1:2359370/71‑1 (MQ=255) aCTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCAt > 1:3169102/1‑46 (MQ=255) aCTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCAt > 1:2526828/1‑46 (MQ=255) aCTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACt > 1:2031864/1‑71 (MQ=255) aCTGTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGa > 1:1978970/1‑41 (MQ=255) aCTGTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTa > 1:362770/1‑61 (MQ=255) aCTGTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTa > 1:3390782/1‑61 (MQ=255) gTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGt > 1:1050674/1‑35 (MQ=255) gTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCt < 1:555357/50‑1 (MQ=255) gTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCt < 1:2988180/50‑1 (MQ=255) gTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCt < 1:2476131/50‑1 (MQ=255) gTTTGCCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGc > 1:3917361/1‑71 (MQ=255) tttGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATcc < 1:2302039/40‑1 (MQ=255) | CCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGC > W3110S.gb/2953762‑2953896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |