| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,971,531 | G→T | 22.2% | L330I (CTA→ATA) | ygeD ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,971,531 | 0 | G | T | 22.2% | 64.5 / 6.6 | 27 | L330I (CTA→ATA) | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (6/15); new base T (2/4); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
TCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCT > W3110S.gb/2971467‑2971598 | tCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACg < 1:3409901/70‑1 (MQ=255) gCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCa < 1:3574314/70‑1 (MQ=255) gCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCa < 1:627546/70‑1 (MQ=255) gCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCa < 1:1654915/70‑1 (MQ=255) aaGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGa < 1:373285/71‑1 (MQ=255) tttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCAt > 1:1654258/1‑63 (MQ=255) cTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACg < 1:437875/54‑1 (MQ=255) gCAATCGCGTTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCaa > 1:1218043/1‑50 (MQ=255) gCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCaa > 1:2052929/1‑50 (MQ=255) cAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaa < 1:3225231/71‑1 (MQ=255) cAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACaaa < 1:1032154/70‑1 (MQ=255) cAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACaaa < 1:2253973/70‑1 (MQ=255) cAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACaaa < 1:1556481/70‑1 (MQ=255) aaTCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaa < 1:1295337/70‑1 (MQ=255) tCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGc < 1:1369050/49‑1 (MQ=255) gCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaa > 1:2943817/1‑66 (MQ=255) ccccgccccgACGCTTTTTTTACCCCGCTCCTGTAGCAACGCAtt > 1:62771/1‑45 (MQ=255) cccgccccgACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCAt < 1:1488914/71‑1 (MQ=255) aCGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGcc < 1:3089846/68‑1 (MQ=255) cGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATc < 1:2661652/71‑1 (MQ=255) gCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAAt > 1:2079699/1‑69 (MQ=255) aCCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATcaac < 1:2153621/70‑1 (MQ=255) ccccGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATcaac < 1:1130279/69‑1 (MQ=255) ccGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg < 1:1058094/71‑1 (MQ=255) gCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCa < 1:1256204/71‑1 (MQ=255) gTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCt > 1:3563030/1‑71 (MQ=255) gTAGCAACGCATTGAGCGGAAAGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCt > 1:879904/1‑71 (MQ=255) | TCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCT > W3110S.gb/2971467‑2971598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |