| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 199,072 | T→C | 58.7% | V382A (GTC→GCC) | yaeT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 199,072 | 0 | T | C | 58.7% | 29.8 / 49.2 | 46 | V382A (GTC→GCC) | yaeT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/9); new base C (8/19); total (18/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACC > W3110S.gb/199004‑199127 | cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCg < 1:3141411/71‑1 (MQ=255)cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCg < 1:3305660/71‑1 (MQ=255) cGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGCTCTGGTCg > 1:335480/1‑69 (MQ=255) gAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATTTGGTCGAt > 1:116244/1‑70 (MQ=255) tGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTa < 1:185740/71‑1 (MQ=255) tCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCg < 1:821189/57‑1 (MQ=255) tCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCg < 1:2043927/57‑1 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAgg > 1:2777869/1‑61 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAgg > 1:3542604/1‑61 (MQ=255) gCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTc > 1:2056040/1‑71 (MQ=255) gCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTc > 1:825180/1‑71 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAgg < 1:3054077/62‑1 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGa < 1:2526037/71‑1 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGa < 1:557250/71‑1 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGa < 1:2952367/71‑1 (MQ=255) gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAAt > 1:566637/1‑71 (MQ=255) cgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATc < 1:3214170/71‑1 (MQ=255) aaaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTa < 1:3267281/54‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc > 1:3442649/1‑71 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:939414/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:41702/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:3121557/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:96865/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:3079477/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:3060032/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGCGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc > 1:3302851/1‑71 (MQ=255) aaTGCGTCAGATGGAAGGGTCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGGCTGAATCGTc < 1:3016131/71‑1 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAg > 1:3204499/1‑54 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAg > 1:806068/1‑54 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAAt > 1:1853500/1‑63 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAAt > 1:748382/1‑63 (MQ=255) gTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCa < 1:3109259/43‑1 (MQ=255) gTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCa < 1:2810632/43‑1 (MQ=255) tCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCg < 1:2734532/63‑1 (MQ=255) tCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCg < 1:258305/63‑1 (MQ=255) aGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGcttc < 1:1900454/71‑1 (MQ=255) gAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTg > 1:697887/1‑70 (MQ=255) gAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGa < 1:3955798/71‑1 (MQ=255) aaGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGa < 1:1147767/70‑1 (MQ=255) gTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACt > 1:1858320/1‑71 (MQ=255) tGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTg > 1:398577/1‑71 (MQ=255) gCATGGCTGGGGAGCGATCTGGCCGATCAGGGTaa > 1:1073560/1‑35 (MQ=255) cATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTc < 1:1148621/71‑1 (MQ=255) gCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata > 1:728452/1‑71 (MQ=255) gggAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgaaacc < 1:1953517/69‑1 (MQ=255) gAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGctt < 1:186949/48‑1 (MQ=255) | CTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACC > W3110S.gb/199004‑199127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |