| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,983,818 | A→G | 33.6% | V144A (GTG→GCG) | yqeF ← | predicted acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,983,818 | 0 | A | G | 33.6% | 129.1 / 109.5 | 113 | V144A (GTG→GCG) | yqeF | predicted acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (41/34); new base G (24/14); total (65/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
TTCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTG > W3110S.gb/2983748‑2983884 | ttCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGca > 1:2907078/1‑71 (MQ=255) gcgAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa < 1:3117661/71‑1 (MQ=255) aGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGAc < 1:2682141/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTc < 1:479040/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGAc > 1:1637454/1‑67 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGt > 1:165983/1‑70 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGt > 1:1363698/1‑70 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:2262017/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:2722201/71‑1 (MQ=255) gATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCA‑AAGACTGTc < 1:2284452/69‑1 (MQ=255) ttttCGGCGGTGACGCCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaac > 1:3797908/1‑71 (MQ=255) ttttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaac > 1:2994413/1‑71 (MQ=255) ttttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaac > 1:119355/1‑71 (MQ=255) tttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaac > 1:1260458/1‑70 (MQ=255) tttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaac > 1:1477966/1‑70 (MQ=255) tttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaac > 1:750677/1‑70 (MQ=255) ggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGc < 1:189585/71‑1 (MQ=255) gACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:4063354/1‑71 (MQ=255) gACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:3349506/1‑71 (MQ=255) acacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAtt > 1:2373367/1‑71 (MQ=255) cacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCt < 1:3555991/67‑1 (MQ=255) acCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaaccaa > 1:1206287/1‑60 (MQ=255) cAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaaccaac > 1:1121281/1‑59 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGa > 1:2784905/1‑46 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:3323871/51‑1 (MQ=255) atatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAg < 1:2846523/44‑1 (MQ=255) atatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAg < 1:841246/44‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa < 1:2953072/42‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:3416775/49‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:76276/49‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:2695797/49‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:1061106/49‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGc > 1:317569/1‑71 (MQ=255) tatGATAATCATTGAAGGCATCCAACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTACCAAGc > 1:1936586/1‑71 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCtg < 1:3687320/71‑1 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCtg > 1:90736/1‑71 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGa > 1:632463/1‑42 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGa > 1:973931/1‑42 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGa > 1:1370889/1‑42 (MQ=255) tGATAATCATTGAAGGCAT‑‑CACAACCCATCATGCACAAGa > 1:1517598/1‑40 (MQ=37) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAcc < 1:2332220/71‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAcc < 1:243413/71‑1 (MQ=255) cATTGAAGGCATCCCACAACCCATGATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCt > 1:649255/1‑63 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaaccaac > 1:674334/1‑46 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaaccaac > 1:3312011/1‑46 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaaccaac > 1:35714/1‑46 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaaccaac > 1:3512746/1‑46 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCgg > 1:2242616/1‑71 (MQ=255) aTTGAAGGCATACCACAACCCATCATGCGCAAGACTGTcaaccaac > 1:3521370/1‑46 (MQ=255) tGAAGGCATCCCACAACCCATCATGCGCAAGACTGTcaaccaac > 1:1565742/1‑44 (MQ=255) tGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTg > 1:918119/1‑46 (MQ=255) tGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTg > 1:1196214/1‑46 (MQ=255) tGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTg > 1:558607/1‑46 (MQ=255) cATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTa < 1:881861/71‑1 (MQ=255) cATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTa < 1:3380673/71‑1 (MQ=255) cATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTa < 1:2858700/71‑1 (MQ=255) aTCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAcc < 1:3263090/60‑1 (MQ=255) tCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGc < 1:1008025/51‑1 (MQ=255) tCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGc < 1:3836633/51‑1 (MQ=255) tCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGc < 1:403405/51‑1 (MQ=255) tCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGc < 1:101407/51‑1 (MQ=255) tCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGc < 1:1180811/67‑1 (MQ=255) ccACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:3886287/1‑71 (MQ=255) ccACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:1965465/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGc > 1:365140/1‑38 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGc > 1:2944602/1‑38 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCaa > 1:909933/1‑47 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCaa > 1:405880/1‑47 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCt > 1:2311364/1‑50 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCTGTGCGGCTAtcag > 1:2724061/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:3229689/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:2496611/1‑71 (MQ=255) ccACAACCCAACATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:2760779/1‑71 (MQ=255) acaACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtgtg < 1:1055762/51‑1 (MQ=255) acaACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtgtg < 1:3684989/51‑1 (MQ=255) aaCCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag < 1:2601645/71‑1 (MQ=255) aaCCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag < 1:734802/71‑1 (MQ=255) cccATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag < 1:3142270/69‑1 (MQ=255) catcatGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGaa < 1:185175/69‑1 (MQ=255) tcatGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtg > 1:2152672/1‑71 (MQ=255) tcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtg < 1:1387900/71‑1 (MQ=255) atGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:4084698/1‑71 (MQ=255) atGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:1289057/1‑71 (MQ=255) atGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:1492092/1‑71 (MQ=255) atGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:1649153/70‑1 (MQ=255) atGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:1529927/70‑1 (MQ=255) atGCGCAAGACTGTCAACCAACTGCCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:2171398/70‑1 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:2092094/1‑58 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:2206419/1‑58 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGaa > 1:3170218/1‑65 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGaa > 1:3724045/1‑65 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:3943970/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:2572015/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:3560144/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:1075491/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:214102/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:2079225/70‑1 (MQ=255) tGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:1680714/69‑1 (MQ=255) acaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:3223414/66‑1 (MQ=255) acaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:3524383/66‑1 (MQ=255) | TTCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTG > W3110S.gb/2983748‑2983884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |