| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,989,906 | A→G | 30.4% | intergenic (+250/‑18) | ygeF → / → ygeG | hypothetical protein/predicted chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,989,906 | 0 | A | G | 30.4% | 150.2 / 95.8 | 112 | intergenic (+250/‑18) | ygeF/ygeG | hypothetical protein/predicted chaperone |
| Reads supporting (aligned to +/- strand): ref base A (37/41); new base G (17/17); total (54/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
CATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATC > W3110S.gb/2989838‑2989975 | cATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGAcaca > 1:695487/1‑71 (MQ=255)cATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGAcaca > 1:1755067/1‑71 (MQ=255) aTAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGacac < 1:854978/69‑1 (MQ=255) aTAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCAt < 1:3072800/71‑1 (MQ=255) aaTTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATca > 1:1358447/1‑71 (MQ=255) tttAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATcaca < 1:2678955/71‑1 (MQ=255) tAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATcaca > 1:2816393/1‑69 (MQ=255) aaaaaGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACag < 1:2622229/68‑1 (MQ=255) gATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAatat < 1:3593165/71‑1 (MQ=255) aaaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAAtattat > 1:3680939/1‑71 (MQ=255) aaaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCAAGAGACGCATCACAGAGGAAtattat > 1:2571867/1‑71 (MQ=255) aaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:4018339/64‑1 (MQ=255) aaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:295361/64‑1 (MQ=255) aaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:2978588/64‑1 (MQ=255) aaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:842820/64‑1 (MQ=255) aaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:2478178/64‑1 (MQ=255) aaTATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATg < 1:3651865/71‑1 (MQ=255) tataAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGaca > 1:440131/1‑49 (MQ=255) ataAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAg > 1:1465392/1‑70 (MQ=255) ataAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAg > 1:486916/1‑70 (MQ=255) taAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGca < 1:3369688/71‑1 (MQ=255) aaaaTATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGca > 1:182441/1‑69 (MQ=255) aaTATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGa < 1:3483135/71‑1 (MQ=255) aaTATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGa < 1:1130121/71‑1 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat < 1:3286244/57‑1 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat < 1:2520817/57‑1 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat < 1:1060766/57‑1 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACTGAGGaa < 1:2933/52‑1 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGaa < 1:3558472/52‑1 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAAc > 1:233607/1‑70 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAAtatta > 1:45522/1‑57 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAAtatta > 1:562938/1‑57 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAAc > 1:1295629/1‑70 (MQ=255) aaTGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAAt > 1:251242/1‑71 (MQ=255) aaTGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAAt > 1:1399545/1‑71 (MQ=255) tGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAAtt > 1:310612/1‑70 (MQ=255) gAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAatat < 1:30177/50‑1 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGaa < 1:880900/71‑1 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaa < 1:2803770/71‑1 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaa < 1:1427873/71‑1 (MQ=255) tcaatcaaTAATAATTTGGCATCACGAGACACATCACAGAGGAatat < 1:1805515/47‑1 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAatat > 1:2132943/1‑71 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAatat > 1:520303/1‑71 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAatat > 1:2524686/1‑71 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAata > 1:2487842/1‑70 (MQ=255) tcaatAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGa < 1:2831485/64‑1 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:2039625/40‑1 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc < 1:2069132/71‑1 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc < 1:1095058/71‑1 (MQ=255) caatAATAATTTGGCATCACGAGACACATCACAGAGGa > 1:278774/1‑38 (MQ=255) caatAATAATTTGGCATCACGAGACACATCACAGAGGa > 1:1482478/1‑38 (MQ=255) caatAATAATTTGGCATCACGAGACACATCACAGAGGa > 1:2972545/1‑38 (MQ=255) caatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc > 1:3279786/1‑70 (MQ=255) aataataatTTGGCATCACGAGACGCATCACAGAGGAAtatta > 1:1924896/1‑43 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAata > 1:2535991/1‑40 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACaa > 1:2695792/1‑58 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:4007645/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:3107017/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:3709864/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:127260/1‑71 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATg < 1:3069199/43‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATg < 1:2335489/43‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATg < 1:3089645/43‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATg < 1:713164/43‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:1940966/62‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:3532512/62‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:2169601/62‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:1964211/62‑1 (MQ=255) aataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa > 1:1626740/1‑71 (MQ=255) ataatTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa > 1:1341695/1‑70 (MQ=255) ataatTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa > 1:1107079/1‑70 (MQ=255) ataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAg > 1:2778721/1‑43 (MQ=255) ataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa < 1:2115436/70‑1 (MQ=255) taatTTGGCATCACGAGACACATCACAGAGGAATATTCTGAGCACAGAAACAATTGAAATATTc < 1:865206/64‑1 (MQ=255) aatTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAg > 1:2667595/1‑71 (MQ=255) aatTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAg > 1:506077/1‑71 (MQ=255) atTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAg > 1:3001378/1‑70 (MQ=255) tttGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:1490207/56‑1 (MQ=255) tttGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:1329408/56‑1 (MQ=255) tttGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:3918614/56‑1 (MQ=255) gCATCAGGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:2596427/70‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:1985080/70‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:29170/70‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:3378639/70‑1 (MQ=255) tCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAAt < 1:3602492/44‑1 (MQ=255) tCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTTATGAATg < 1:752963/71‑1 (MQ=255) cACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAata > 1:3267254/1‑50 (MQ=255) aCGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATggg > 1:774134/1‑71 (MQ=255) aCGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATggg > 1:470130/1‑71 (MQ=255) aCGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATggg > 1:2096181/1‑71 (MQ=255) aCGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATggg > 1:2351245/1‑71 (MQ=255) aCGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATggg > 1:2540527/1‑71 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGaa > 1:2976815/1‑67 (MQ=255) gagaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCa < 1:37903/71‑1 (MQ=255) gagaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCa < 1:2673847/71‑1 (MQ=255) agaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCa > 1:1995745/1‑70 (MQ=255) agaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCa > 1:2346743/1‑70 (MQ=255) acacATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGt < 1:3470652/56‑1 (MQ=255) acacATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGt < 1:2977771/56‑1 (MQ=255) cacaTCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATc < 1:1088807/71‑1 (MQ=255) | CATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATC > W3110S.gb/2989838‑2989975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |