| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,992,923 | C→T | 25.6% | pseudogene (227/375 nt) | pbl → | ECK2852:JW5457:b2854; predicted peptidoglycan‑binding enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,992,923 | 0 | C | T | 25.6% | 157.8 / 59.1 | 90 | pseudogene (227/375 nt) | pbl | ECK2852:JW5457:b2854; predicted peptidoglycan‑binding enzyme |
| Reads supporting (aligned to +/- strand): ref base C (19/47); new base T (9/14); total (29/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
AAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGG > W3110S.gb/2992856‑2992992 | aaaCGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCga < 1:2639683/71‑1 (MQ=255) tgtaataatAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGAttt < 1:3481345/68‑1 (MQ=255) tGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGAttt > 1:859336/1‑71 (MQ=255) ggAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGAttt > 1:3903994/1‑70 (MQ=255) gAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAATCGAGATTTta < 1:2628538/71‑1 (MQ=255) ataaAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATaa > 1:4018937/1‑71 (MQ=255) ataaAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATaa > 1:633640/1‑71 (MQ=255) aaaaGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAAc > 1:3376514/1‑70 (MQ=255) aaaaGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAAc > 1:3666247/1‑70 (MQ=255) aaaaGATGAACGAGATTTGTTAGATAACCCTTGGCTGAAGATTTAAATAGGAATCgag < 1:1257198/58‑1 (MQ=255) aaGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCAt < 1:3212331/71‑1 (MQ=255) gATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCAttt < 1:1488711/71‑1 (MQ=255) tGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCAttttt > 1:569876/1‑71 (MQ=255) tGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCga < 1:1776392/51‑1 (MQ=255) tGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCga < 1:3456/51‑1 (MQ=255) tGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCAttttt < 1:892741/71‑1 (MQ=255) tGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCAttttt < 1:1400740/71‑1 (MQ=255) tGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCAttttt > 1:1986533/1‑71 (MQ=255) gagaTTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGt > 1:3737757/1‑71 (MQ=255) aTTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCga < 1:1713099/43‑1 (MQ=255) aTTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCga < 1:2775406/43‑1 (MQ=255) aTTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCAc < 1:908790/66‑1 (MQ=255) tttGTTAGATAACCCTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:1284907/71‑1 (MQ=255) tttGTTAGATAACCCTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:2024537/71‑1 (MQ=255) tttGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:2554336/71‑1 (MQ=255) ttGTTAGATAACCCTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTtgtg > 1:3924766/1‑70 (MQ=255) ttGTTAGATAACCCTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTtgtg > 1:1472134/1‑70 (MQ=255) ttGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGt > 1:1303366/1‑66 (MQ=255) tGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg > 1:1204128/1‑69 (MQ=255) aTAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:3118256/63‑1 (MQ=255) aTAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:1982032/63‑1 (MQ=255) aTAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:341307/63‑1 (MQ=255) aaCCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTg > 1:852311/1‑71 (MQ=255) cccTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:2283887/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:2593497/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:3394910/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:1163851/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:3842544/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:3877050/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:1952267/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:1942402/71‑1 (MQ=255) cccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:14527/71‑1 (MQ=255) ccTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:1438263/70‑1 (MQ=255) ccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:3337436/58‑1 (MQ=255) ccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTaa > 1:3492039/1‑64 (MQ=255) ccTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:857702/70‑1 (MQ=255) cTTGTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCaa < 1:1490041/71‑1 (MQ=255) cTTGTCTGAACATTAAAATAGGAACCGAGATTTta < 1:840247/35‑1 (MQ=255) cTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGa > 1:2292441/1‑59 (MQ=255) cTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCaa < 1:2680660/71‑1 (MQ=255) cTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCaa < 1:3381499/71‑1 (MQ=255) gTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATg < 1:964266/70‑1 (MQ=255) gTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGc > 1:3926043/1‑71 (MQ=255) gTCTGAACATTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGc > 1:2222113/1‑71 (MQ=255) gTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAAt < 1:735309/69‑1 (MQ=255) gTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGc > 1:2192288/1‑71 (MQ=255) gTCTGAACATTAAAATAGGAAACGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGc > 1:2395228/1‑71 (MQ=255) tCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:2219602/53‑1 (MQ=255) cTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:3230032/52‑1 (MQ=255) cTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTtgtg < 1:1364036/52‑1 (MQ=255) aaCATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTggg > 1:3777978/1‑71 (MQ=255) aaCATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTggg > 1:2880151/1‑71 (MQ=255) aTTAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:818692/58‑1 (MQ=255) ttAAAATAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACt < 1:689885/71‑1 (MQ=255) ttAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGt < 1:1294067/41‑1 (MQ=255) ttAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGt < 1:227747/41‑1 (MQ=255) ttAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGt < 1:469902/41‑1 (MQ=255) tAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACt > 1:1839036/1‑70 (MQ=255) aaaaTAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGa > 1:419373/1‑45 (MQ=255) aaaaTAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTta > 1:1709963/1‑71 (MQ=255) aaaaTAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTta > 1:489150/1‑71 (MQ=255) aaaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGcc > 1:2481209/1‑61 (MQ=255) aaaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTta > 1:1144833/1‑71 (MQ=255) aaaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTta > 1:2966949/1‑71 (MQ=255) aaaTAGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTtat < 1:3665599/71‑1 (MQ=255) aaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTtat < 1:2125309/71‑1 (MQ=255) aaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTtat < 1:2365276/71‑1 (MQ=255) aaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTtat < 1:3777402/71‑1 (MQ=255) aaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTtat < 1:2418794/71‑1 (MQ=255) aaaTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTtat < 1:1483504/71‑1 (MQ=255) aTAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATaa < 1:3277813/71‑1 (MQ=255) aGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:4058096/50‑1 (MQ=255) aGGAATCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGc < 1:2472952/50‑1 (MQ=255) aaCCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGcc < 1:3147748/53‑1 (MQ=255) aaCCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGcc < 1:3507509/53‑1 (MQ=255) aaCCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGcc < 1:1204134/53‑1 (MQ=255) aaCCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGcc < 1:597607/53‑1 (MQ=255) aaCCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGcc < 1:3099476/53‑1 (MQ=255) aaCCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGcc < 1:4039584/53‑1 (MQ=255) aCCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCggg < 1:280215/71‑1 (MQ=255) | AAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGG > W3110S.gb/2992856‑2992992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |