| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,993,568 | C→A | 25.4% | intergenic (‑9/+25) | ygeK ← / ← ygeL | predicted DNA‑binding transcriptional regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,993,568 | 0 | C | A | 25.4% | 113.6 / 19.1 | 47 | intergenic (‑9/+25) | ygeK/ygeL | predicted DNA‑binding transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (15/20); new base A (7/5); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
ATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCA > W3110S.gb/2993504‑2993633 | ataataTGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCaaa < 1:2290656/71‑1 (MQ=255) atatGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATtc < 1:509075/71‑1 (MQ=255) atatGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAAtt > 1:2838512/1‑70 (MQ=255) tGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTcc > 1:375068/1‑71 (MQ=255) tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:916358/1‑70 (MQ=255) tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:3885763/1‑70 (MQ=255) tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:3350505/1‑70 (MQ=255) tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:2370894/1‑70 (MQ=255) tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:2258999/1‑70 (MQ=255) atctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa > 1:1212702/1‑71 (MQ=255) atctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAAtt > 1:970417/1‑58 (MQ=255) tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:1077825/70‑1 (MQ=255) tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataa < 1:3110198/69‑1 (MQ=255) tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataa < 1:1412918/69‑1 (MQ=255) tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAaaataaa < 1:391321/70‑1 (MQ=255) ctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata < 1:820296/67‑1 (MQ=255) cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAAtt > 1:1338947/1‑50 (MQ=255) cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAgg > 1:3414988/1‑71 (MQ=255) cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAaataaata > 1:2063690/1‑65 (MQ=255) cGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAaataaata > 1:3703676/1‑65 (MQ=255) tttAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:1165534/71‑1 (MQ=255) tttAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:2778673/71‑1 (MQ=255) tttAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:3681277/71‑1 (MQ=255) aCCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTa < 1:1556324/69‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:2126233/51‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg < 1:2168986/62‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTTTAGATTTGt < 1:847010/71‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTTTAGATTTGt < 1:632370/71‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:2253906/71‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:3667456/71‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:3151944/71‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:637533/71‑1 (MQ=255) ttACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa > 1:4018395/1‑71 (MQ=255) ttACGAGTTCGGCCCCCATCATTCCACACGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa > 1:2623176/1‑71 (MQ=255) gTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:1101330/43‑1 (MQ=255) gTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaataaa < 1:1840041/43‑1 (MQ=255) ccATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCt < 1:2274598/71‑1 (MQ=255) atcatTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGa > 1:1401652/1‑47 (MQ=255) tcatTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa < 1:3783547/53‑1 (MQ=255) catTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttct > 1:4057697/1‑71 (MQ=255) atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:1254659/1‑71 (MQ=255) atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:1249926/1‑71 (MQ=255) atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:3501801/1‑71 (MQ=255) atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:919866/1‑71 (MQ=255) ttCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTg < 1:4027305/47‑1 (MQ=255) ccACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa > 1:351514/1‑48 (MQ=255) ccACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCa < 1:1371553/70‑1 (MQ=255) | ATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCA > W3110S.gb/2993504‑2993633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |