| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,053,087 | T→A | 29.7% | Y137F (TAT→TTT) | pepP ← | proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,053,087 | 0 | T | A | 29.7% | 70.4 / 35.9 | 47 | Y137F (TAT→TTT) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base T (14/19); new base A (8/6); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTGAT > W3110S.gb/3053020‑3053157 | tgcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg < 1:1885360/71‑1 (MQ=255) cggtgAGATTTTGCCGCGTACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca < 1:1948994/71‑1 (MQ=255) cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:3094693/1‑71 (MQ=255) cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:1548510/1‑71 (MQ=255) cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:1622733/1‑71 (MQ=255) cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgca > 1:3752664/1‑71 (MQ=255) tttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcata > 1:1355928/1‑58 (MQ=255) tttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGccc > 1:475115/1‑71 (MQ=255) tttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGccc > 1:1926650/1‑71 (MQ=255) ttGACGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTAGCCCt > 1:2512147/1‑71 (MQ=255) cgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg < 1:3565007/53‑1 (MQ=255) ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGt > 1:3418881/1‑70 (MQ=255) ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTa < 1:3147513/71‑1 (MQ=255) ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTa < 1:3234897/71‑1 (MQ=255) tttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTggg < 1:2259841/62‑1 (MQ=255) tACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTaaa > 1:2698254/1‑69 (MQ=255) aGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATc < 1:455364/71‑1 (MQ=255) aGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTg > 1:2047341/1‑53 (MQ=255) tttttCCAGCGCACTGTTCACGATTACATCAgcat < 1:320915/35‑1 (MQ=255) tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:1629379/70‑1 (MQ=255) tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:354897/70‑1 (MQ=255) tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:3841753/70‑1 (MQ=255) tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:502776/70‑1 (MQ=255) tttatCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:2558724/70‑1 (MQ=255) tttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATc > 1:1394703/1‑67 (MQ=255) tttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATc > 1:2810377/1‑67 (MQ=255) tttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAgg > 1:3085803/1‑71 (MQ=255) tttCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAgg > 1:3329839/1‑71 (MQ=255) aGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGtt < 1:3500674/71‑1 (MQ=255) aGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGtt < 1:350992/71‑1 (MQ=255) gcgcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACca < 1:2759713/57‑1 (MQ=255) gcgcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACca < 1:3857668/57‑1 (MQ=255) gcgcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACca < 1:2079452/57‑1 (MQ=255) cgcACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGtt > 1:255326/1‑69 (MQ=255) gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAag < 1:3785778/71‑1 (MQ=255) gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAag < 1:959456/71‑1 (MQ=255) aCTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagta < 1:3523494/71‑1 (MQ=255) aCTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagta < 1:2075116/71‑1 (MQ=255) cTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTaa < 1:2510110/48‑1 (MQ=255) cTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATc < 1:3788474/56‑1 (MQ=255) tGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATc < 1:1979979/55‑1 (MQ=255) gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGtt > 1:2145686/1‑71 (MQ=255) gTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCa > 1:2363465/1‑56 (MQ=255) gTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCa > 1:2598623/1‑56 (MQ=255) cACGATTACATCAGCATATGCATATTCGCCCTGGGCa > 1:1813313/1‑37 (MQ=255) cACGATTACATCAGCATATGCATATTCGCCCTGGGCa > 1:3744824/1‑37 (MQ=255) aCGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATa < 1:3171134/71‑1 (MQ=255) cGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATaa < 1:335302/71‑1 (MQ=255) aTTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagtag < 1:2624329/62‑1 (MQ=255) aTTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagtag < 1:1726709/62‑1 (MQ=255) tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa > 1:3378213/1‑56 (MQ=255) tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa > 1:2245411/1‑56 (MQ=255) tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa > 1:1247852/1‑56 (MQ=255) tACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGtt > 1:3078735/1‑62 (MQ=255) tCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt > 1:1477368/1‑70 (MQ=255) tCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt > 1:260719/1‑70 (MQ=255) catatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgatt < 1:55340/70‑1 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATc < 1:3071427/35‑1 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATc < 1:419773/35‑1 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATCGAGGCCGTTAAGTAGTTGATAAAGTTGCt < 1:87917/64‑1 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:2458983/1‑71 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:2902148/1‑71 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:664345/1‑71 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattgat > 1:3060571/1‑71 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt < 1:2225784/64‑1 (MQ=255) tatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt < 1:1626926/64‑1 (MQ=255) | TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTGAT > W3110S.gb/3053020‑3053157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |