| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,062,130 | A→C | 38.7% | E163A (GAA→GCA) | argK → | membrane ATPase/protein kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,062,130 | 0 | A | C | 38.7% | ‑3.1 / 40.9 | 31 | E163A (GAA→GCA) | argK | membrane ATPase/protein kinase |
| Reads supporting (aligned to +/- strand): ref base A (10/9); new base C (6/6); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGG > W3110S.gb/3062063‑3062196 | ttATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGa < 1:436594/68‑1 (MQ=255)ttATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGa < 1:1927726/68‑1 (MQ=255) aTGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCg > 1:3763101/1‑71 (MQ=255) aTGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCg > 1:3352658/1‑71 (MQ=255) aTGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCg > 1:2248249/1‑71 (MQ=255) gcagcGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAt < 1:1158452/71‑1 (MQ=255) gcagcGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAt < 1:2981712/71‑1 (MQ=255) cGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAtggtg < 1:2915076/71‑1 (MQ=255) ttATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACt < 1:3288598/71‑1 (MQ=255) ttATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACt < 1:1996877/71‑1 (MQ=255) ttATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACt > 1:3190870/1‑71 (MQ=255) tGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAtg < 1:737664/53‑1 (MQ=255) tGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAtg < 1:3316575/53‑1 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCAtggt > 1:59752/1‑53 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCAtggt > 1:297889/1‑53 (MQ=255) ttGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCAtg < 1:357920/50‑1 (MQ=255) tGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTg > 1:3078889/1‑70 (MQ=255) tCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCaa < 1:1742985/71‑1 (MQ=255) gTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAAt < 1:1300371/65‑1 (MQ=255) gTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAAt < 1:1745628/65‑1 (MQ=255) gCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCgat > 1:1965270/1‑70 (MQ=255) gCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTTTTTTATCTCGTTGCAAATTGCCGGTGGCGGCgatg < 1:135571/71‑1 (MQ=255) aGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCgatgat < 1:3558915/71‑1 (MQ=255) gTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCgatg < 1:4009994/68‑1 (MQ=255) tCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTggcggc < 1:680522/63‑1 (MQ=255) cGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCgatgat > 1:1675978/1‑68 (MQ=255) cGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCgatgat > 1:3793033/1‑68 (MQ=255) cGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATc > 1:1769235/1‑69 (MQ=255) cGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATc > 1:2016547/1‑69 (MQ=255) ggAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa > 1:3764107/1‑35 (MQ=255) ggAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa > 1:3212094/1‑35 (MQ=255) aCAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAggg > 1:589657/1‑71 (MQ=255) aCAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAggg > 1:594453/1‑71 (MQ=255) | TTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGG > W3110S.gb/3062063‑3062196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |