| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,065,306 | A→G | 24.1% | V175A (GTT→GCT) | ygfI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,065,306 | 0 | A | G | 24.1% | 96.3 / 32.4 | 54 | V175A (GTT→GCT) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (17/24); new base G (5/8); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
CGCATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGA > W3110S.gb/3065240‑3065376 | cgcATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacgag < 1:3185468/71‑1 (MQ=255) ccAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacg < 1:486315/58‑1 (MQ=255) ccAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacg < 1:481452/58‑1 (MQ=255) ccAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacg < 1:1860068/58‑1 (MQ=255) cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCg > 1:716923/1‑71 (MQ=255) cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:168785/69‑1 (MQ=255) cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTa < 1:927684/71‑1 (MQ=255) aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAg < 1:365879/69‑1 (MQ=255) aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:34662/71‑1 (MQ=255) cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:605714/1‑38 (MQ=255) cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:2811443/1‑38 (MQ=255) cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:2328345/1‑38 (MQ=255) cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:3574101/1‑38 (MQ=255) ctcttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:801021/71‑1 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:2773614/1‑69 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:2837375/1‑56 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:2808103/1‑56 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:1939757/1‑69 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:2992657/1‑69 (MQ=255) cttgctattgTCGCTCTTCATAACTGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:2297293/1‑56 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtgg < 1:749165/57‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAg > 1:3856635/1‑71 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAAt < 1:804983/46‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGc < 1:3782959/49‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:3006012/52‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:1682911/52‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:519911/52‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAg > 1:3258299/1‑71 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:1559097/1‑52 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:1492519/1‑52 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:2564006/1‑52 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:2630869/1‑52 (MQ=255) tcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:3216757/58‑1 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc < 1:3859931/57‑1 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc < 1:1087096/57‑1 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc < 1:182942/57‑1 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:2414129/1‑71 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTaa > 1:2895098/1‑37 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGTTGGTAGTTCACTGATAGTCAgg < 1:1529836/68‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:3662455/68‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:3251922/68‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:1813381/68‑1 (MQ=255) ttCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa < 1:2106199/55‑1 (MQ=255) ttCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa < 1:3219454/55‑1 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:3348613/37‑1 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:3638801/37‑1 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:2272175/37‑1 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:1884287/47‑1 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCa < 1:2354817/49‑1 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc < 1:230168/67‑1 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:346334/1‑37 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:2065775/1‑71 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:1718274/1‑71 (MQ=255) aaTAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:618847/2‑65 (MQ=255) tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:3958962/68‑1 (MQ=255) tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:1293108/68‑1 (MQ=255) tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:1512101/68‑1 (MQ=255) tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg < 1:2928512/71‑1 (MQ=255) tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTACAGTTCCTg < 1:3635070/71‑1 (MQ=255) aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAg > 1:3991367/1‑63 (MQ=255) aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAg > 1:766193/1‑63 (MQ=255) aCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc > 1:3900177/1‑66 (MQ=255) agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:3182154/71‑1 (MQ=255) agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:2970435/71‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:3890914/71‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:300048/71‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:291700/71‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:2400100/71‑1 (MQ=255) aacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc < 1:2439935/52‑1 (MQ=255) acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa < 1:2095658/71‑1 (MQ=255) acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa < 1:1903108/71‑1 (MQ=255) | CGCATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGA > W3110S.gb/3065240‑3065376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |