| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 14,173 | T→C | 19.6% | A2A (GCT→GCC) | dnaJ → | chaperone Hsp40, co‑chaperone with DnaK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 14,173 | 0 | T | C | 19.6% | 117.3 / 25.8 | 56 | A2A (GCT→GCC) | dnaJ | chaperone Hsp40, co‑chaperone with DnaK |
| Reads supporting (aligned to +/- strand): ref base T (23/22); new base C (9/2); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
GGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGC > W3110S.gb/14112‑14238 | ggCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat < 1:3448436/71‑1 (MQ=255)ggCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCCAAGCAAGat > 1:794248/1‑71 (MQ=255) cGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGatta < 1:1102303/71‑1 (MQ=255) aGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAttatta > 1:1681196/1‑71 (MQ=255) gAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgag < 1:621422/71‑1 (MQ=255) aTTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCCAAGCAAGATTATTACGAGAt > 1:1535975/1‑71 (MQ=255) tttCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGatt < 1:1977082/60‑1 (MQ=255) tCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAttt > 1:3776451/1‑70 (MQ=255) tCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAttt < 1:2345396/70‑1 (MQ=255) tcCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCCAAGCAAGATTATTACGAGATTTTAGGCg > 1:1546995/1‑71 (MQ=255) gTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAttt < 1:3964040/58‑1 (MQ=255) gTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAttt < 1:2731251/58‑1 (MQ=255) gTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAttt < 1:2198692/58‑1 (MQ=255) cATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat < 1:2562901/42‑1 (MQ=255) cATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa > 1:182097/1‑70 (MQ=255) cATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa > 1:3895327/1‑70 (MQ=255) ttCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAg > 1:1372735/1‑71 (MQ=255) tCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat > 1:2386719/1‑39 (MQ=255) tCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat > 1:3875258/1‑39 (MQ=255) cATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa > 1:1260598/1‑53 (MQ=255) cATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa > 1:2043603/1‑53 (MQ=255) cATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa > 1:2345826/1‑53 (MQ=255) cATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa > 1:899531/1‑53 (MQ=255) cATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg > 1:685020/1‑55 (MQ=255) cATCTAGGGGCAATTTAAAAAAGATGGCCAAGCAAGatta > 1:303842/1‑40 (MQ=255) aTCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCgg < 1:3772927/71‑1 (MQ=255) tCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCgg < 1:2230792/70‑1 (MQ=255) tAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgaga > 1:2378490/1‑44 (MQ=255) tAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgaga > 1:537717/1‑44 (MQ=255) tAGGGGCAATTTAAAAAAGATGGCCAAGCAAGattat > 1:826376/1‑37 (MQ=255) cAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTg < 1:2532025/71‑1 (MQ=255) tttAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa < 1:2266422/51‑1 (MQ=255) tttAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa < 1:2241061/51‑1 (MQ=255) tttAAAAAAGATGGCCAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCgg < 1:2152643/59‑1 (MQ=255) ttAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt > 1:315393/1‑71 (MQ=255) ttAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt > 1:348139/1‑71 (MQ=255) ttAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt > 1:2234669/1‑71 (MQ=255) ttAAAAAAGATGGCCAAGCAAGATTATTACGAGATTTTAGGCGTTTCCa > 1:2781868/1‑49 (MQ=255) tAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa > 1:3521206/1‑38 (MQ=255) tAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa > 1:2362470/1‑38 (MQ=255) tAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa > 1:2615203/1‑51 (MQ=255) tAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt > 1:1398469/1‑70 (MQ=255) tAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt < 1:3783522/70‑1 (MQ=255) tAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt > 1:1536474/1‑70 (MQ=255) tAAAAAAGATGGCCAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt > 1:3728996/1‑70 (MQ=255) tAAAAAAGATGGCCAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt > 1:3858625/1‑70 (MQ=255) aaaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGa < 1:3198778/71‑1 (MQ=255) aaaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGa < 1:4033047/71‑1 (MQ=255) aaaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGa < 1:195660/71‑1 (MQ=255) aaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAg < 1:1241630/49‑1 (MQ=255) aaGATGGCCAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGa > 1:811724/1‑69 (MQ=255) aGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAg < 1:3601413/71‑1 (MQ=255) aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGa < 1:929106/66‑1 (MQ=255) aTGGCCAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGc < 1:1434114/71‑1 (MQ=255) tGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGa < 1:1743550/65‑1 (MQ=255) ggCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGaaa > 1:551222/1‑66 (MQ=255) | GGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGC > W3110S.gb/14112‑14238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |