| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,093,680 | G→A | 23.2% | H19H (CAC→CAT) | yggR ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,093,680 | 0 | G | A | 23.2% | 111.1 / 30.5 | 56 | H19H (CAC→CAT) | yggR | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (20/23); new base A (7/6); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
GCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCG > W3110S.gb/3093613‑3093749 | gCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGatg > 1:3966305/1‑70 (MQ=255) cGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGgtg > 1:1364564/1‑69 (MQ=255) aaaCGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATcc > 1:965476/1‑70 (MQ=255) aaaCGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATcc > 1:3925524/1‑70 (MQ=255) ggCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGAc < 1:1227698/71‑1 (MQ=255) gcAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGt > 1:275783/1‑70 (MQ=255) cAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTa < 1:2298683/71‑1 (MQ=255) cTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGAc > 1:3775223/1‑64 (MQ=255) cTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGc > 1:1838548/1‑71 (MQ=255) ccATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCtt < 1:3758980/70‑1 (MQ=255) cATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTa < 1:1285972/71‑1 (MQ=255) ttCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGATGTa < 1:3362186/48‑1 (MQ=255) tCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTaca > 1:1047673/1‑70 (MQ=255) cTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCg > 1:240521/1‑52 (MQ=255) cTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTacac > 1:842960/1‑70 (MQ=255) cTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTacac > 1:941934/1‑70 (MQ=255) cTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTacac > 1:496531/1‑70 (MQ=255) ccGCGAATGCGCCATCGTGCGGGCCAGTCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTa < 1:2718303/69‑1 (MQ=255) ccGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTaa < 1:2221154/70‑1 (MQ=255) ccGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTa < 1:1553776/69‑1 (MQ=255) ccGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTa < 1:1189812/69‑1 (MQ=255) aaTGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACt < 1:3664371/63‑1 (MQ=255) aaTGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACt < 1:3412712/63‑1 (MQ=255) gcCATCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTACACTAGGGGCCACAAtt < 1:2552110/71‑1 (MQ=255) aTCGTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCtt > 1:1965003/1‑49 (MQ=255) tCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCtt < 1:2550902/48‑1 (MQ=255) tCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca < 1:515728/64‑1 (MQ=255) cGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCgaga > 1:3937113/1‑37 (MQ=255) gTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTaca > 1:1617514/1‑50 (MQ=255) gTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTa > 1:1474771/1‑48 (MQ=255) gTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATttcttc > 1:1116181/1‑70 (MQ=255) gTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATttcttc < 1:919765/70‑1 (MQ=255) gTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATttcttc > 1:3266589/1‑70 (MQ=255) gTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATttcttc < 1:3069419/70‑1 (MQ=255) gTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTaca > 1:118672/1‑50 (MQ=255) gTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTaca > 1:123320/1‑50 (MQ=255) gTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTaca > 1:2953695/1‑50 (MQ=255) gTGCGGGCCAGGCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTaca > 1:1620731/1‑50 (MQ=255) tGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATttcttc < 1:331953/69‑1 (MQ=255) ccAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCa < 1:1772783/71‑1 (MQ=255) ccAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCa < 1:412553/71‑1 (MQ=255) ccAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACAAAGGGCCACAATTTCTTCCATATTCa < 1:2965210/71‑1 (MQ=255) cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTa < 1:249112/45‑1 (MQ=255) cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTa < 1:3521975/45‑1 (MQ=255) cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTa < 1:271433/45‑1 (MQ=255) cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCa > 1:2721625/1‑70 (MQ=255) cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCa > 1:3809154/1‑70 (MQ=255) cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCa > 1:3345059/1‑70 (MQ=255) cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCa > 1:3114483/1‑70 (MQ=255) ggCGCTGCACAGATGTAGATCCGAGACGTTATGCTTTa < 1:1479659/38‑1 (MQ=255) gcgcTGCACAGGTGTAGATCCGAGACTTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCa < 1:3204620/67‑1 (MQ=255) gcgcTGCACAGGTGTAGATCCGAGACGTTATGCTTTa < 1:3752290/37‑1 (MQ=255) gcgcTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACt < 1:4063448/71‑1 (MQ=255) cgcTGCACAGATGTAGATCCGAGACGTTATGCTTTaca > 1:2833430/1‑38 (MQ=255) cacaGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCAt < 1:3531741/62‑1 (MQ=255) cacaGATGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCAt < 1:4004982/62‑1 (MQ=255) aGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTc > 1:3474315/1‑71 (MQ=255) aGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTc > 1:2906188/1‑71 (MQ=255) ggTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCg > 1:1877060/1‑71 (MQ=255) gtgtAGATCCGAGACGTTATGCTTTACACTAAGGGCcaca < 1:2512214/40‑1 (MQ=255) gtgtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCg > 1:2828312/1‑70 (MQ=255) gtgtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCg > 1:1465862/1‑70 (MQ=255) gtgtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATACTCg > 1:3638455/1‑70 (MQ=255) | GCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCG > W3110S.gb/3093613‑3093749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |