| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,100,981 | A→G | 27.8% | H176H (CAT→CAC) | yggH ← | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,100,981 | 0 | A | G | 27.8% | 51.2 / 24.4 | 36 | H176H (CAT→CAC) | yggH | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (13/13); new base G (7/3); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGC > W3110S.gb/3100911‑3101048 | ttttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCata > 1:929591/1‑71 (MQ=255)ttttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCata > 1:3882947/1‑71 (MQ=255)ttttATAACCGTCAATAGAAGACAACACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCata > 1:2373503/1‑71 (MQ=255) ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTgg < 1:2733294/70‑1 (MQ=255) ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:1992487/70‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCata > 1:1618086/1‑66 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:1429355/69‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:1618134/71‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:1660282/71‑1 (MQ=255) tCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCata < 1:2084566/60‑1 (MQ=255) tCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCata < 1:681814/60‑1 (MQ=255) aaTAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGccc > 1:3196197/1‑70 (MQ=255) aaTAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGccc > 1:304128/1‑70 (MQ=255) aaTAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGccc > 1:2969672/1‑70 (MQ=255) gaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:2885066/1‑59 (MQ=255) gaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:2736054/1‑59 (MQ=255) gaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:3352342/56‑1 (MQ=255) tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCa < 1:4058236/69‑1 (MQ=255) tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCACCCCAGCTGCa < 1:3990705/69‑1 (MQ=255) aCTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATg > 1:996124/1‑47 (MQ=255) aCTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGttt > 1:2538186/1‑71 (MQ=255) aCTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGttt > 1:736977/1‑71 (MQ=255) cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:1211595/1‑46 (MQ=255) cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:1685457/1‑46 (MQ=255) cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:2553721/1‑46 (MQ=255) cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:2469189/1‑46 (MQ=255) cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGc < 1:28055/69‑1 (MQ=255) cAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCtt > 1:209440/1‑71 (MQ=255) aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCtttt < 1:856415/71‑1 (MQ=255) aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCtttt < 1:71109/71‑1 (MQ=255) tatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:6262/38‑1 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGAATa > 1:1061368/1‑40 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAg > 1:1361766/1‑71 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAg > 1:1356978/1‑71 (MQ=255) ccGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCg > 1:3438567/1‑71 (MQ=255) aGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAAc < 1:3520073/70‑1 (MQ=255) gTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGc < 1:1052707/71‑1 (MQ=255) cAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCttttt > 1:3384085/1‑48 (MQ=255) ggtcgCCATGTGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:237818/71‑1 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAg > 1:569585/1‑48 (MQ=255) gCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACg > 1:1489647/1‑71 (MQ=255) cATGTGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGc < 1:1875705/71‑1 (MQ=255) | TTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGC > W3110S.gb/3100911‑3101048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |