| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,104,160 | C→T | 29.0% | R358C (CGC→TGC) | mltC → | membrane‑bound lytic murein transglycosylase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,104,160 | 0 | C | T | 29.0% | 42.7 / 20.7 | 31 | R358C (CGC→TGC) | mltC | membrane‑bound lytic murein transglycosylase C |
| Reads supporting (aligned to +/- strand): ref base C (10/12); new base T (5/4); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
TCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCAC > W3110S.gb/3104103‑3104221 | tctGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGAt < 1:1929754/64‑1 (MQ=255) tGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCa < 1:2141716/69‑1 (MQ=255) tGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCAt > 1:2340059/1‑70 (MQ=255) cGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTa > 1:2179644/1‑70 (MQ=255) cGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCAtt < 1:2606745/69‑1 (MQ=255) tctctcCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAAc > 1:3833106/1‑67 (MQ=255) tctcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACAGCCGCCGATAATTCCATTAAc > 1:3887477/1‑67 (MQ=255) tcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGcccc > 1:648892/1‑71 (MQ=255) ttATCTTTATAAAGTGAATACCGCGCAAAAATCCTAccgccgcc < 1:1034176/44‑1 (MQ=255) tATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGc > 1:314273/1‑61 (MQ=255) aTCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACGATg > 1:1164208/1‑71 (MQ=255) aTCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGa > 1:1809515/1‑69 (MQ=255) aTCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATg > 1:3892490/1‑71 (MQ=255) ttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCa > 1:3649161/1‑48 (MQ=255) ttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCa > 1:345605/1‑48 (MQ=255) tataAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAAc > 1:434876/1‑52 (MQ=255) taAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAgg < 1:2723878/70‑1 (MQ=255) tGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaa < 1:307362/71‑1 (MQ=255) aaTACCGCGCAAAAATCCTACCGCTGCCGATAATTCCa > 1:578312/1‑38 (MQ=255) aaTACCGCGCAAAAATCCTACCGCTGCCGATAATTCCa > 1:825166/1‑38 (MQ=255) aaTACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaaa < 1:3710903/70‑1 (MQ=255) aaTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaaa > 1:2422711/1‑70 (MQ=255) aTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTc < 1:220845/59‑1 (MQ=255) aTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTc < 1:1578021/59‑1 (MQ=255) aTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTc < 1:1316167/59‑1 (MQ=255) ccGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGt < 1:1457705/70‑1 (MQ=255) gcAAAAATCCTACCGCCTCCGATAATTCCATTAACCGCCCCTGAc < 1:365164/45‑1 (MQ=255) aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAc < 1:32952/42‑1 (MQ=255) aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTc < 1:1577424/49‑1 (MQ=255) tCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATcac < 1:2723253/71‑1 (MQ=255) tCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATcac < 1:2718581/71‑1 (MQ=255) tCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATcac < 1:1960314/71‑1 (MQ=255) | TCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCAC > W3110S.gb/3104103‑3104221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |