| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,128,408 | T→C | 33.4% | D155G (GAC→GGC) | yghO ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,128,408 | 0 | T | C | 33.4% | 46.1 / 40.8 | 46 | D155G (GAC→GGC) | yghO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (13/17); new base C (10/5); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
CCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTA > W3110S.gb/3128342‑3128473 | cccAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaaa < 1:1776679/71‑1 (MQ=255) aGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCTTCATCGCACAGGGTGGTGTGTCAAAAcc > 1:623287/1‑71 (MQ=255) aGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAAcc > 1:3539717/1‑71 (MQ=255) aGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAAcc > 1:799994/1‑71 (MQ=255) tgttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCtt > 1:1723879/1‑70 (MQ=255) tgttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCtt > 1:3813304/1‑70 (MQ=255) tgttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCtt > 1:320988/1‑70 (MQ=255) tatGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAg > 1:3181967/1‑70 (MQ=255) cgcgGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGgtgtgt > 1:3999564/1‑50 (MQ=255) cgcgGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGgtgtgt > 1:383929/1‑50 (MQ=255) cgcgGCATACCACGGTTTGCCGTGTGCCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATc < 1:2762090/71‑1 (MQ=255) cATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCtt < 1:541845/71‑1 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTcc < 1:304191/70‑1 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTcc < 1:2994320/70‑1 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:2480924/1‑71 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:3938280/1‑71 (MQ=255) cACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtg < 1:828963/71‑1 (MQ=255) aCGGTTTGCCGTGTGGCATCATCGCACAGGGTGgtgtgt > 1:3526874/1‑39 (MQ=255) aCGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTaa > 1:1940294/1‑58 (MQ=255) aCGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtga > 1:1646654/1‑71 (MQ=255) aCGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtga > 1:1384907/1‑71 (MQ=255) gTTTGCCGTGTGGCATCATCG‑‑CAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtgatt > 1:1053158/1‑68 (MQ=255) gCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGata < 1:3427147/70‑1 (MQ=255) tgtgGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAg < 1:1215144/71‑1 (MQ=255) gCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCt > 1:1596923/1‑70 (MQ=255) atcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:2921785/71‑1 (MQ=255) tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:2483839/51‑1 (MQ=255) tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:3980456/51‑1 (MQ=255) tcatcGCACAGGGTGGTGTGGCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:1387962/51‑1 (MQ=255) atcGCAGAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg > 1:523828/1‑70 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgat > 1:2123484/1‑52 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtga > 1:884721/1‑51 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg > 1:1863046/1‑70 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg > 1:3688340/1‑70 (MQ=255) atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATc > 1:3857043/1‑40 (MQ=255) atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg > 1:3383146/1‑70 (MQ=255) atcGCACAGGGT‑GTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtga > 1:3511569/1‑50 (MQ=39) cGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGAc > 1:1855888/1‑71 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCttt < 1:4072750/40‑1 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCttt < 1:405881/40‑1 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCttt < 1:4044453/40‑1 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCttt < 1:2165596/40‑1 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCttt < 1:2506359/40‑1 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCtt < 1:956813/39‑1 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCtt < 1:2312630/39‑1 (MQ=255) caGGGTGGTGTGTCAAAACCTTCAATCAGGAATCCGCttt < 1:328031/40‑1 (MQ=255) aGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTg < 1:3302415/71‑1 (MQ=255) ggtggtGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:2614750/40‑1 (MQ=255) tggtgTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATAtt < 1:868447/48‑1 (MQ=255) gtgtgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTa < 1:381596/71‑1 (MQ=255) gtgtgcCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga > 1:2131900/1‑42 (MQ=255) tCAAAACCTTCAATCAGTAATACGCTTTCCTGATTGatat > 1:2688339/1‑40 (MQ=255) | CCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTA > W3110S.gb/3128342‑3128473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |