| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,141,409 | C→T | 28.6% | A79A (GCG→GCA) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,141,409 | 0 | C | T | 28.6% | 87.9 / 40.7 | 56 | A79A (GCG→GCA) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (23/17); new base T (3/13); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
GCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCAC > W3110S.gb/3141342‑3141477 | gCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCTCGTTAACCGGAACGTCGATATTCAGCGCa > 1:3066549/1‑71 (MQ=255)gCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCa > 1:38989/1‑71 (MQ=255) cGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCa < 1:3318166/70‑1 (MQ=255) gtggtgTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGctct < 1:3944111/71‑1 (MQ=255) gtggtgTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACt < 1:206333/71‑1 (MQ=255) gtggtgTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACt < 1:1826507/71‑1 (MQ=255) tgtgCGCAGCCAGAATGATGTTACGGATTTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCt < 1:1564323/71‑1 (MQ=255) tgtgCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCt < 1:715083/71‑1 (MQ=255) cgcAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCg > 1:3868178/1‑71 (MQ=255) gCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACg < 1:965545/71‑1 (MQ=255) gCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACg < 1:1916544/71‑1 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACgg < 1:183271/71‑1 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg < 1:1913173/71‑1 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACg > 1:2526207/1‑70 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTAGATATTCAGCGCACTTTCTGCCGCACGAACgg < 1:131425/71‑1 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:2656165/1‑69 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:2598945/1‑69 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:2842338/71‑1 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:2659611/71‑1 (MQ=255) gatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:910236/1‑68 (MQ=255) gatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:1684776/1‑68 (MQ=255) gatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:3649702/70‑1 (MQ=255) tgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCt < 1:1108980/53‑1 (MQ=255) aCGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgcg > 1:2741870/1‑71 (MQ=255) aCGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgc > 1:721700/1‑70 (MQ=255) tGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACt < 1:665202/40‑1 (MQ=255) tATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGt < 1:1568077/71‑1 (MQ=255) tATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGt < 1:3381152/71‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa > 1:2710161/1‑55 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:1151767/1‑69 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:3209269/1‑69 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:159574/1‑69 (MQ=255) cgcgTTAACCGGAACGTCGATATTCAGCGCACTTTCTGcag > 1:3105811/1‑39 (MQ=255) cgcgTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAg > 1:3857124/1‑65 (MQ=255) ttAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACgg < 1:2487899/46‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACgg < 1:1757990/46‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa < 1:2316352/71‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa < 1:1245332/71‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa < 1:544668/71‑1 (MQ=255) tAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACgg < 1:505628/45‑1 (MQ=255) aaCCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGaa > 1:2189148/1‑46 (MQ=255) aaCCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgta > 1:690787/1‑64 (MQ=255) aaCCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgta > 1:70025/1‑64 (MQ=255) gTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGa < 1:240579/71‑1 (MQ=255) ctCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGa < 1:738940/70‑1 (MQ=255) gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATAc > 1:494058/1‑71 (MQ=255) gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATAc < 1:3840185/71‑1 (MQ=255) gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATAc > 1:817801/1‑71 (MQ=255) atatTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATAc < 1:3419331/70‑1 (MQ=255) tatTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGa < 1:756591/66‑1 (MQ=255) atTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGtt > 1:4966/1‑71 (MQ=255) atTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGtt > 1:1979164/1‑71 (MQ=255) atTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGtt > 1:2565729/1‑71 (MQ=255) cAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGt > 1:3199180/1‑67 (MQ=255) cAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGca > 1:815239/1‑71 (MQ=255) aGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGcac < 1:1476752/71‑1 (MQ=255) | GCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCAC > W3110S.gb/3141342‑3141477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |