| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,163,647 | T→A | 35.7% | N328I (AAC→ATC) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,163,647 | 0 | T | A | 35.7% | 67.8 / 74.7 | 70 | N328I (AAC→ATC) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base T (32/13); new base A (10/15); total (42/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
CATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCA > W3110S.gb/3163581‑3163713 | cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTaa < 1:2542209/70‑1 (MQ=255) cGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa > 1:1106550/1‑71 (MQ=255) cGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa > 1:2446128/1‑71 (MQ=255) cGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa > 1:152992/1‑71 (MQ=255) cGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGa > 1:2428/1‑71 (MQ=255) ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa < 1:153936/70‑1 (MQ=255) ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa < 1:548762/70‑1 (MQ=255) ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa < 1:682503/70‑1 (MQ=255) ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa < 1:3269505/70‑1 (MQ=255) gagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGc > 1:2574068/1‑71 (MQ=255) gATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGATAGctct > 1:1532457/1‑71 (MQ=255) gATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGATAGctct > 1:2375115/1‑71 (MQ=255) tttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATa > 1:435637/1‑59 (MQ=255) tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTc > 1:3437433/1‑71 (MQ=255) agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAg < 1:3472445/71‑1 (MQ=255) agGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGATAGctc < 1:1561771/60‑1 (MQ=255) gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATAc < 1:2462114/50‑1 (MQ=255) gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCtttt < 1:1500088/62‑1 (MQ=255) gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCtttt < 1:3634170/62‑1 (MQ=255) tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAg < 1:3255308/65‑1 (MQ=255) ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTaa > 1:2906306/1‑45 (MQ=255) ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTaa > 1:3980229/1‑45 (MQ=255) ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATa > 1:3469424/1‑52 (MQ=255) ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAg > 1:3722742/1‑53 (MQ=255) ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGa > 1:3619305/1‑50 (MQ=255) ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGa > 1:374707/1‑50 (MQ=255) tCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAAt < 1:3255752/43‑1 (MQ=255) tCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAAt < 1:668254/43‑1 (MQ=255) gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTACAGATCGGTGGTAGCGAAGAgg < 1:3212287/71‑1 (MQ=255) gacgacCATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg < 1:345917/71‑1 (MQ=255) cgacCATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtg < 1:3054557/71‑1 (MQ=255) ccATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAagag < 1:1450087/65‑1 (MQ=255) ccATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAagag < 1:3146792/65‑1 (MQ=255) ccATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAagag < 1:2459712/65‑1 (MQ=255) ccATCCAGACCGATCATATTAAGGATAATACGATACCTCTTTTCCAGATCGGTGGTAGCGAagag < 1:683085/65‑1 (MQ=255) aTCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggt > 1:2765792/1‑65 (MQ=255) aTCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggt > 1:270959/1‑65 (MQ=255) tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcat > 1:3245768/1‑71 (MQ=255) tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcat > 1:2482526/1‑71 (MQ=255) tCCAGACCGATCATATTAAGGATAATACGATAGctc < 1:1037372/36‑1 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCttt > 1:883089/1‑37 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCttt > 1:3163571/1‑37 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCttt > 1:1615432/1‑37 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca > 1:3678862/1‑71 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca > 1:157286/1‑71 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca > 1:3707009/1‑71 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca > 1:2510182/1‑71 (MQ=255) cAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 1:1930854/57‑1 (MQ=255) aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa > 1:2996999/1‑54 (MQ=255) aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa > 1:2312212/1‑54 (MQ=255) aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa > 1:3049681/1‑54 (MQ=255) aCCGATCATATTAAGGTTAATACGATAGCTCTTTTACAGATCGGTGGTAGCGaa > 1:2246092/1‑54 (MQ=255) ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtggt > 1:3831786/1‑61 (MQ=255) ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCt > 1:2016900/1‑70 (MQ=255) tCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggt < 1:3398175/40‑1 (MQ=255) atatTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa > 1:1444053/1‑70 (MQ=255) atatTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa > 1:2434355/1‑70 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCggt > 1:509646/1‑35 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa > 1:2026637/1‑44 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 1:3633197/44‑1 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa > 1:492480/1‑44 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa > 1:3889742/1‑44 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 1:3749875/44‑1 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc > 1:3892844/1‑71 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc > 1:2794102/1‑71 (MQ=255) ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc > 1:3029823/1‑71 (MQ=255) ttAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAag < 1:3793864/45‑1 (MQ=255) ttAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAag < 1:3720985/45‑1 (MQ=255) ttAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAag < 1:237800/45‑1 (MQ=255) aGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCa > 1:497043/1‑70 (MQ=255) ttAATACGATAGCTCTTTTCCAGATCGGTGGTAGCg < 1:2389516/36‑1 (MQ=255) | CATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCA > W3110S.gb/3163581‑3163713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |