| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,204,990 | T→C | 45.0% | intergenic (‑78/‑129) | ygiP ← / → ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,204,990 | 0 | T | C | 45.0% | 12.0 / 80.7 | 60 | intergenic (‑78/‑129) | ygiP/ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (18/15); new base C (18/9); total (36/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACA > W3110S.gb/3204921‑3205058 | aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATattcatt > 1:2940278/1‑71 (MQ=255)aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATattcat > 1:2468061/1‑70 (MQ=255)aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATattcat > 1:1047083/1‑70 (MQ=255) gtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTcg > 1:3609067/1‑71 (MQ=255) tAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGtt < 1:1687335/71‑1 (MQ=255) aaGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGtt > 1:1074734/1‑70 (MQ=255) aaGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGtt > 1:1410494/1‑70 (MQ=255) aaGCGCAGTGTATTTCGCAAAACATTGCCATTCCCCGCTCTGGCGCAATATTCACTCTTATTTTCGCGtt > 1:184662/1‑70 (MQ=255) aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa > 1:3191411/1‑71 (MQ=255) gcgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAt < 1:2276380/71‑1 (MQ=255) cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTAtt < 1:3470660/59‑1 (MQ=255) gcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAtt < 1:2260586/70‑1 (MQ=255) gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACg < 1:583133/71‑1 (MQ=255) gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACg < 1:3675968/71‑1 (MQ=255) gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACg < 1:2920316/71‑1 (MQ=255) gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACg < 1:1185309/71‑1 (MQ=255) gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACg < 1:3846199/71‑1 (MQ=255) tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAttt < 1:3230368/55‑1 (MQ=255) gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa > 1:3083187/1‑63 (MQ=255) gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGaa > 1:1499033/1‑71 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATattcatt > 1:1148045/1‑44 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCActct > 1:3332595/1‑46 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCActct > 1:2690450/1‑46 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTTGCGTTg < 1:321060/59‑1 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGa > 1:1525926/1‑60 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGa > 1:2934718/1‑60 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGa > 1:1241073/1‑60 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGt > 1:264187/1‑70 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGt > 1:1849262/1‑70 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGt > 1:1212342/1‑70 (MQ=255) tCGCAAAACATTCCCATTCCCTCCTCTGGCGCAATATTCATACAAATTAACGCGTAGATTCACGAACTg > 1:1850292/1‑69 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:1663629/70‑1 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAAt < 1:2109295/71‑1 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAAt < 1:6274/71‑1 (MQ=255) aCATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACa < 1:1049087/71‑1 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCAc < 1:318408/71‑1 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCAc < 1:861835/71‑1 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCAc < 1:1338995/71‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGtt < 1:432642/40‑1 (MQ=255) taccTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGAtt > 1:3901933/3‑43 (MQ=38) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCa > 1:425856/1‑44 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCa > 1:2905603/1‑44 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCa > 1:3080834/1‑44 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGa > 1:981018/1‑47 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:3253228/1‑55 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:3599423/1‑55 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAc > 1:2198620/1‑59 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACata > 1:1865564/1‑62 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTg > 1:920543/1‑71 (MQ=255) cccTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTg > 1:3547278/1‑71 (MQ=255) cccTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTg > 1:3725887/1‑71 (MQ=255) cccTGCTCTGGCACAATATTCATTCTTATTTTCGCGTTGATTCACGa > 1:1408216/1‑47 (MQ=255) tGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGt > 1:52322/1‑49 (MQ=255) tGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGt > 1:991937/1‑49 (MQ=255) tGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGt > 1:2214278/1‑49 (MQ=255) gCTCTGGCGAAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:3654705/1‑51 (MQ=255) ctctGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCaa > 1:3173033/1‑70 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAAt > 1:2845110/1‑49 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAatat < 1:612772/71‑1 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAatat < 1:166253/71‑1 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAatat < 1:437549/71‑1 (MQ=255) gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAatat > 1:2002998/1‑70 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAata > 1:3878915/1‑66 (MQ=255) atatTCATTCTTATTTTCGCGTTCATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAata > 1:1152730/1‑66 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTa < 1:1971386/42‑1 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAatat < 1:2550216/66‑1 (MQ=255) attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACgg > 1:2990548/1‑52 (MQ=255) attcactcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAAc > 1:590572/1‑70 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaaaa > 1:732708/1‑68 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaaaa > 1:1278051/1‑68 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaaaa > 1:2792476/1‑68 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:1038235/66‑1 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:1932335/66‑1 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACca > 1:2584012/1‑71 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACca > 1:3443732/1‑71 (MQ=255) ttcactcTTATTTTCGCGTTGATTCACGAACTGTTAATTACata > 1:2529512/1‑44 (MQ=255) ttcactcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACca > 1:1801649/1‑71 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTgg > 1:767668/1‑53 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTgg > 1:1477485/1‑53 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACcaca > 1:2672692/1‑71 (MQ=255) | ATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACA > W3110S.gb/3204921‑3205058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |