| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,222,206 | C→T | 8.8% | N306N (AAC→AAT) | ebgA → | cryptic beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,222,206 | 0 | C | T | 8.8% | 208.7 / 8.6 | 68 | N306N (AAC→AAT) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/28); new base T (4/2); total (38/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATC > W3110S.gb/3222138‑3222275 | cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:953953/71‑1 (MQ=255)cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:2680331/71‑1 (MQ=255)cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:2052336/71‑1 (MQ=255) agcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:1494918/70‑1 (MQ=255) aaTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACCCTGAAAGACGCCAACGGCAACGTTCTg > 1:4080999/1‑71 (MQ=255) aTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTgg < 1:2234930/71‑1 (MQ=255) aTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGACAACGTTCTgg < 1:2876455/71‑1 (MQ=255) gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCttg > 1:206929/1‑66 (MQ=255) gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTgg > 1:1311610/1‑68 (MQ=255) gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTgg > 1:232290/1‑68 (MQ=255) gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAg > 1:1180702/1‑71 (MQ=255) agAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGtt > 1:936254/1‑58 (MQ=255) aaTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt < 1:1420708/64‑1 (MQ=255) aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:2637517/71‑1 (MQ=255) aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGT‑GTGCcac > 1:1190634/1‑70 (MQ=255) tCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac > 1:2858473/1‑70 (MQ=255) ccccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:2593799/1‑59 (MQ=255) cccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGtt > 1:3124564/1‑52 (MQ=255) cccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:1758822/1‑58 (MQ=255) cccTTATCTTTACCATCAGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:3474959/1‑58 (MQ=255) ttATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAAcgcg > 1:1569892/1‑71 (MQ=255) tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCATCGTTCTGGAATTGGTGCCACAAcgc < 1:144542/69‑1 (MQ=255) tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGt > 1:3454655/1‑71 (MQ=255) tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGt > 1:289194/1‑71 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt > 1:1753755/1‑71 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:1749313/1‑53 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgtgg > 1:2325323/1‑57 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGtt > 1:1342195/1‑71 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGtt > 1:2573641/1‑71 (MQ=255) tCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTg > 1:810656/1‑71 (MQ=255) cTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:139027/61‑1 (MQ=255) tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGc > 1:2721121/1‑71 (MQ=255) tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGc > 1:1178421/1‑71 (MQ=255) tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGCAAGTGGTGCCACAACGCGTTGGc > 1:652854/1‑71 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTTGTGCCACAACGCGTTGGCTTCCGt < 1:744023/71‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:1810736/71‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:927378/71‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:2264431/71‑1 (MQ=255) tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt > 1:2258338/1‑70 (MQ=255) gTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa < 1:3305433/68‑1 (MQ=255) gTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACTCGTTGGCTTCCGTg < 1:3385898/67‑1 (MQ=255) tCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt < 1:1817340/40‑1 (MQ=255) tCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt < 1:763325/40‑1 (MQ=255) cATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATc > 1:1274095/1‑70 (MQ=255) aTGACGCTGAAAGACGCTAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa < 1:2549169/70‑1 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:2763314/46‑1 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:942999/46‑1 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:938134/46‑1 (MQ=255) tGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa < 1:4060533/69‑1 (MQ=255) gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg > 1:4082907/1‑71 (MQ=255) gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg > 1:3156714/1‑71 (MQ=255) gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg > 1:50206/1‑71 (MQ=255) aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACaa > 1:431877/1‑45 (MQ=255) aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaaa > 1:3683716/1‑69 (MQ=255) aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaaa > 1:3508892/1‑69 (MQ=255) aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaaa > 1:2089465/1‑69 (MQ=255) aaGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGAc > 1:3509061/1‑71 (MQ=255) aaGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGAc > 1:3197849/1‑71 (MQ=255) ccAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa < 1:3269500/49‑1 (MQ=255) ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa < 1:3283618/54‑1 (MQ=255) ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTg < 1:3575399/71‑1 (MQ=255) ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTg < 1:1833628/71‑1 (MQ=255) ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTg < 1:2385274/71‑1 (MQ=255) aaCGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGt > 1:3061718/1‑70 (MQ=255) aCGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTc < 1:181728/71‑1 (MQ=255) ggCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGt > 1:1211826/1‑63 (MQ=255) aaCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGtctggtctgg > 1:229196/1‑69 (MQ=255) aCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATc < 1:3181671/71‑1 (MQ=255) | CAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATC > W3110S.gb/3222138‑3222275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |